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Literature summary for 2.5.1.29 extracted from

  • Dogbo, O.; Camara, B.
    Purification of isopentenyl pyrophosphate isomerase and geranylgeranyl pyrophosphate synthase from Capsicum chromoplasts by affinity chromatography (1987), Biochim. Biophys. Acta, 920, 140-148.
No PubMed abstract available
Search for more literature for 2.5.1.29

Inhibitors

Inhibitors Comment Organism Structure
additional information inhibition by irradiation with the photolabile analog of geranyl diphosphate Capsicum annuum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00095
-
 dimethylallyl diphosphate
-
 Capsicum annuum
0.001
-
 geranyl diphosphate
-
 Capsicum annuum
0.0012
-
 farnesyl diphosphate
-
 Capsicum annuum
0.003
-
 isopentenyl diphosphate
-
 Capsicum annuum

Localization

Localization Comment Organism GeneOntology No. Textmining
chromoplast stroma Capsicum annuum 9509
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 2 * 37000, SDS-PAGE Capsicum annuum
74000
-
 gel filtration Capsicum annuum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate Capsicum annuum
-
 geranylgeranyl diphosphate + diphosphate
-
 ?
dimethylallyl diphosphate + 3 isopentenyl diphosphate Capsicum annuum
-
 geranylgeranyl diphosphate + 3 diphosphate
-
 ?
geranyl diphosphate + 2 isopentenyl diphosphate Capsicum annuum
-
 geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Capsicum annuum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
poly(ethylene glycol) 6000, DEAE-Sephacel, aminophenethyl diphosphate affinity column Capsicum annuum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.017
-
-
 Capsicum annuum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Capsicum annuum geranylgeranyl diphosphate + diphosphate
-
 ?
dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
 Capsicum annuum geranylgeranyl diphosphate + 3 diphosphate
-
 ?
geranyl diphosphate + 2 isopentenyl diphosphate
-
 Capsicum annuum geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE Capsicum annuum