KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
isopentenyl diphosphate | - |
Phycomyces blakesleeanus | |
0.06 | - |
farnesyl diphosphate | - |
Phycomyces blakesleeanus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, the native enzyme consists of 2 identical subunits with a tendency to dissociate, SDS-PAGE | Phycomyces blakesleeanus |
60000 | - |
gel filtration, enzyme consists of 2 identical subunits of 30000 Da with a tendency to dissociate | Phycomyces blakesleeanus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | Phycomyces blakesleeanus | preferred allylic substrate | geranylgeranyl diphosphate + diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | Phycomyces blakesleeanus | less than 5% of the activity with farnesyl diphosphate | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | Phycomyces blakesleeanus | less than 5% of the activity with farnesyl diphosphate | geranylgeranyl diphosphate + 2 diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phycomyces blakesleeanus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
polyethylene glycol, DE-52 cellulose, DEAE Trisacryl-M, Reactive blue 4-agarose, Reactive red 120-agarose | Phycomyces blakesleeanus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3 | - |
- |
Phycomyces blakesleeanus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | preferred allylic substrate | Phycomyces blakesleeanus | geranylgeranyl diphosphate + diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | less than 5% of the activity with farnesyl diphosphate | Phycomyces blakesleeanus | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | less than 5% of the activity with farnesyl diphosphate | Phycomyces blakesleeanus | geranylgeranyl diphosphate + 2 diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, the native enzyme consists of 2 identical subunits with a tendency to dissociate, SDS-PAGE | Phycomyces blakesleeanus |