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Literature summary for 2.5.1.29 extracted from
- Interaction with the small subunit of geranyl diphosphate synthase modifies the chain length specificity of geranylgeranyl diphosphate synthase to produce geranyl diphosphate (2002), J. Biol. Chem., 277, 3141-3149.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of preprotein and truncated versions in Escherichia coli | Taxus canadensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0026 | - |
farnesyl diphosphate | - |
Taxus canadensis |  |
| 0.0034 | - |
Mg2+ | - |
Taxus canadensis | |
| 0.0077 | - |
geranyl diphosphate | - |
Taxus canadensis | |
| 0.039 | - |
isopentenyl diphosphate | cosubstrate farnesyl diphosphate | Taxus canadensis | |
| 0.088 | - |
isopentenyl diphosphate | cosubstrate dimethylallyl diphosphate | Taxus canadensis | |
| 0.122 | - |
isopentenyl diphosphate | cosubstrate geranyl diphosphate | Taxus canadensis | |
| 0.127 | - |
dimethylallyl diphosphate | - |
Taxus canadensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000, recombinant enzyme, SDS-PAGE | Taxus canadensis |
| 66000 | - |
gel filtration | Taxus canadensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E,E)-farnesyl diphosphate + isopentenyl diphosphate | Abies grandis | - |
geranylgeranyl diphosphate + diphosphate | - |
? | |
| dimethylallyl diphosphate + 3 isopentenyl diphosphate | Abies grandis | - |
geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
| geranyl diphosphate + 2 isopentenyl diphosphate | Abies grandis | - |
geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Abies grandis | Q8W1R9 | - |
- |
| Taxus canadensis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Abies grandis | geranylgeranyl diphosphate + diphosphate | - |
? | |
| dimethylallyl diphosphate + 3 isopentenyl diphosphate | - |
Taxus canadensis | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
| dimethylallyl diphosphate + 3 isopentenyl diphosphate | - |
Abies grandis | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
| farnesyl diphosphate + isopentenyl diphosphate | - |
Taxus canadensis | geranylgeranyl diphosphate + diphosphate | - |
? | |
| geranyl diphosphate + 2 isopentenyl diphosphate | - |
Abies grandis | geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
| geranyl diphosphate + isopentenyl diphosphate | - |
Taxus canadensis | farnesyl diphosphate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 32000, recombinant enzyme, SDS-PAGE | Taxus canadensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
dimethylallyl diphosphate | - |
Taxus canadensis | |
| 2.1 | - |
geranyl diphosphate | - |
Taxus canadensis | |
| 3.3 | - |
farnesyl diphosphate | - |
Taxus canadensis | |
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Release 2023.1 (January 2023)
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