Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Kitasatospora griseola |
Protein Variants | Comment | Organism |
---|---|---|
A57L | mutated enzyme produces mainly farnesyl diphosphate | Kitasatospora griseola |
L50S | similar activity as wild-type | Kitasatospora griseola |
S4F | very weak activity | Kitasatospora griseola |
V8A | similar activity as wild-type | Kitasatospora griseola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
isopentenyl diphosphate | cosubstrate farnesyl diphosphate | Kitasatospora griseola | |
0.066 | - |
dimethylallyl diphosphate | - |
Kitasatospora griseola | |
0.099 | - |
dimethylallyl diphosphate | A57L mutant enzyme | Kitasatospora griseola | |
0.108 | - |
isopentenyl diphosphate | cosubstrate dimethylallyl diphosphate | Kitasatospora griseola | |
0.121 | - |
farnesyl diphosphate | - |
Kitasatospora griseola |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | Kitasatospora griseola | - |
geranylgeranyl diphosphate + diphosphate | - |
? | |
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | Kitasatospora griseola MF730-N6 | - |
geranylgeranyl diphosphate + diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | Kitasatospora griseola | - |
geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | Kitasatospora griseola MF730-N6 | - |
geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | Kitasatospora griseola | - |
geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | Kitasatospora griseola MF730-N6 | - |
geranylgeranyl diphosphate + 2 diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kitasatospora griseola | - |
MF730-N6 | - |
Kitasatospora griseola MF730-N6 | - |
MF730-N6 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Kitasatospora griseola | geranylgeranyl diphosphate + diphosphate | - |
? | |
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Kitasatospora griseola MF730-N6 | geranylgeranyl diphosphate + diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | - |
Kitasatospora griseola | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | - |
Kitasatospora griseola MF730-N6 | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | - |
Kitasatospora griseola | geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | - |
Kitasatospora griseola MF730-N6 | geranylgeranyl diphosphate + 2 diphosphate | - |
? |