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Literature summary for 2.5.1.29 extracted from

  • Kawasaki, T.; Hamano, Y.; Kuzuyama, T.; Itoh, N.; Seto, H.; Dairi, T.
    Interconversion of the product specificity of type I eubacterial farnesyl diphosphate synthase and geranylgeranyl diphosphate synthase through one amino acid substitution (2003), J. Biochem., 133, 83-91.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Kitasatospora griseola

Protein Variants

Protein Variants Comment Organism
A57L mutated enzyme produces mainly farnesyl diphosphate Kitasatospora griseola
L50S similar activity as wild-type Kitasatospora griseola
S4F very weak activity Kitasatospora griseola
V8A similar activity as wild-type Kitasatospora griseola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
isopentenyl diphosphate cosubstrate farnesyl diphosphate Kitasatospora griseola
0.066
-
dimethylallyl diphosphate
-
Kitasatospora griseola
0.099
-
dimethylallyl diphosphate A57L mutant enzyme Kitasatospora griseola
0.108
-
isopentenyl diphosphate cosubstrate dimethylallyl diphosphate Kitasatospora griseola
0.121
-
farnesyl diphosphate
-
Kitasatospora griseola

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate Kitasatospora griseola
-
geranylgeranyl diphosphate + diphosphate
-
?
(E,E)-farnesyl diphosphate + isopentenyl diphosphate Kitasatospora griseola MF730-N6
-
geranylgeranyl diphosphate + diphosphate
-
?
dimethylallyl diphosphate + 3 isopentenyl diphosphate Kitasatospora griseola
-
geranylgeranyl diphosphate + 3 diphosphate
-
?
dimethylallyl diphosphate + 3 isopentenyl diphosphate Kitasatospora griseola MF730-N6
-
geranylgeranyl diphosphate + 3 diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate Kitasatospora griseola
-
geranylgeranyl diphosphate + 2 diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate Kitasatospora griseola MF730-N6
-
geranylgeranyl diphosphate + 2 diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Kitasatospora griseola
-
MF730-N6
-
Kitasatospora griseola MF730-N6
-
MF730-N6
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
Kitasatospora griseola geranylgeranyl diphosphate + diphosphate
-
?
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
Kitasatospora griseola MF730-N6 geranylgeranyl diphosphate + diphosphate
-
?
dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
Kitasatospora griseola geranylgeranyl diphosphate + 3 diphosphate
-
?
dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
Kitasatospora griseola MF730-N6 geranylgeranyl diphosphate + 3 diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate
-
Kitasatospora griseola geranylgeranyl diphosphate + 2 diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate
-
Kitasatospora griseola MF730-N6 geranylgeranyl diphosphate + 2 diphosphate
-
?