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Literature summary for 2.5.1.3 extracted from
- The enzymatic synthesis of thiamine monophosphate (1961), J. Biol. Chem., 236, 3066-3071.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | - |
Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate | pH 9.2, 37°C | Saccharomyces cerevisiae | |
| 0.007 | - |
2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate | pH 9.2, 37°C | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Saccharomyces cerevisiae | |
| Mg2+ | 1 mM, 3-6fold stimulation | Saccharomyces cerevisiae | |
| Mn2+ | half as effective as Mg2+ | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, 6 months, less than 10% loss of activity | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole | - |
Saccharomyces cerevisiae | thiamine monophosphate + diphosphate | - |
? | |
| 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole | - |
Saccharomyces cerevisiae | thiamine monophosphate + diphosphate | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 21 | - |
room temperature, completely inactivated overnight | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.2 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | 10 | about 40% of activity maximum at pH 7.7 and pH 10.0 | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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