Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl phosphate | 4 mM, 82.8% inhibition | Escherichia coli | |
ADP | 4 mM, 42.4% inhibition | Escherichia coli | |
ATP | 4 mM, 64.7% inhibition | Escherichia coli | |
CTP | 4 mM, 58.7% inhibition | Escherichia coli | |
diphosphate | 0.05 mM, 50% inhibition | Escherichia coli | |
GTP | 4 mM, 76.7% inhibition | Escherichia coli | |
phosphocreatine | 4 mM, 63.8% inhibition | Escherichia coli | |
phosphoenolpyruvate | 4 mM, 31.1% inhibition | Escherichia coli | |
UTP | 4 mM, 71.6% inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate | - |
Escherichia coli | |
0.00085 | - |
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates, 20% as effective as Mg2+ | Escherichia coli | |
Co2+ | stimulates, 20% as effective as Mg2+ | Escherichia coli | |
Mg2+ | required | Escherichia coli | |
Mg2+ | Km: 0.063 mM | Escherichia coli | |
Mg2+ | most effective at 6 mM | Escherichia coli | |
Mn2+ | stimulates, at 6 mM equally effective as Mg2+ | Escherichia coli | |
Zn2+ | stimulates, 20% as effective as Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12, strain PT-R1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.065 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, stable for several months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole | - |
Escherichia coli | thiamine monophosphate + diphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
5 min, without addition of both substrates, completely inactivated | Escherichia coli |