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Literature summary for 2.5.1.31 extracted from

  • Allen, C.M.
    Purification and characterization of undecaprenylpyrophosphate synthetase (1985), Methods Enzymol., 110, 281-299.
    View publication on PubMed
Search for more literature for 2.5.1.31

Activating Compound

Activating Compound Comment Organism Structure
cardiolipin weak stimulation Micrococcus luteus
cardiolipin Lactobacillus plantarum, Escherichia coli, and bovine cardiolipin. Good activator in absence of detergent Lactiplantibacillus plantarum
cetyl sulfate half-maximal activation at 0.04 mM Lactiplantibacillus plantarum
deoxycholate weak stimulation Bacillus subtilis
deoxycholate weak stimulation Micrococcus luteus
deoxycholate half-maximal activation at 0.33 mM Lactiplantibacillus plantarum
Dodecyl sulfate half-maximal activation at 0.075 mM Lactiplantibacillus plantarum
lecithin weak stimulation Bacillus subtilis
lecithin from egg, stimulates Micrococcus luteus
oleic acid good activator in absence of detergent Lactiplantibacillus plantarum
phosphatidic acid from egg, good activator in absence of detergent Lactiplantibacillus plantarum
Triton X-100 stimulates Micrococcus luteus
Triton X-100 strong stimulation Bacillus subtilis
Triton X-100 half-maximal activation at 3.4 mM Lactiplantibacillus plantarum
Tween 80 strong stimulation Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
1,2-Cyclohexanedione
-
 Lactiplantibacillus plantarum
Butanedione
-
 Lactiplantibacillus plantarum
iodoacetate
-
 Lactiplantibacillus plantarum
additional information no inhibition by phosphate Lactiplantibacillus plantarum
Phenylglyoxal
-
 Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00013
-
 (E,E)-farnesyl diphosphate pH 7.5, 35°C Lactiplantibacillus plantarum
0.00192
-
 isopentenyl diphosphate pH 7.5, 35°C Lactiplantibacillus plantarum
0.008
-
 (E,E)-farnesyl diphosphate
-
 Micrococcus luteus
0.0091
-
 (E,E)-farnesyl diphosphate
-
 Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required Lactiplantibacillus plantarum
Co2+ divalent cation required Bacillus subtilis
Co2+ divalent cation required Micrococcus luteus
Co2+ enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ Micrococcus luteus
Co2+ enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+ Lactiplantibacillus plantarum
K+ stimulates in presence of Mg2+ Lactiplantibacillus plantarum
K+ stimulates in presence of Mg2+ Bacillus subtilis
Mg2+ divalent cation required Lactiplantibacillus plantarum
Mg2+ divalent cation required Bacillus subtilis
Mg2+ divalent cation required Micrococcus luteus
Mg2+ enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ Micrococcus luteus
Mg2+ enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+ Lactiplantibacillus plantarum
Mg2+ best stimulation by Mg2+. Mn2+ and Zn2+ may partially replace Mn2+ Bacillus subtilis
Mn2+ can partially replace Mg2+ Bacillus subtilis
Mn2+ enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ Micrococcus luteus
Mn2+ enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+ Lactiplantibacillus plantarum
Mn2+ best stimulation by Mg2+. Mn2+ and Zn2+ may partially replace Mn2+ Bacillus subtilis
Na+ stimulates in presence of Mg2+ Lactiplantibacillus plantarum
Na+ stimulates in presence of Mg2+ Bacillus subtilis
NH4+ stimulates in presence of Mg2+ Lactiplantibacillus plantarum
NH4+ stimulates in presence of Mg2+ Bacillus subtilis
Zn2+
-
 Lactiplantibacillus plantarum
Zn2+
-
 Micrococcus luteus
Zn2+ can partially replace Mg2+ Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 2 * 30000, SDS-PAGE Lactiplantibacillus plantarum
52000 58000 sucrose density gradient centrifugation Lactiplantibacillus plantarum
53000 60000 gel filtration Lactiplantibacillus plantarum
56000
-
 gel filtration, sucrose density gradient centrifugation Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lactiplantibacillus plantarum involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components ?
-
 ?
additional information Bacillus subtilis involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components ?
-
 ?
additional information Micrococcus luteus involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Lactiplantibacillus plantarum
-
 ATCC 8014
-
Micrococcus luteus
-
-
-
Salmonella newington
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactiplantibacillus plantarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Bacillus subtilis 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Micrococcus luteus 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
isopentenyl diphosphate + farnesyl diphosphate
-
 Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + farnesyl diphosphate
-
 Salmonella newington C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + farnesyl diphosphate trans,trans-farnesyl diphosphate and cis,trans-farnesyl diphosphate Lactiplantibacillus plantarum C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + farnesyl diphosphate trans,trans-farnesyl diphosphate Lactiplantibacillus plantarum C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + farnesyl diphosphate trans,trans-farnesyl diphosphate Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + geranyl diphosphate
-
 Lactiplantibacillus plantarum C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + geranyl diphosphate
-
 Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + geranyl diphosphate
-
 Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + geranylgeranyl diphosphate
-
 Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + geranylgeranyl diphosphate
-
 Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + geranylgeranyl diphosphate trans,trans,trans-geranylgeranyl diphosphate Lactiplantibacillus plantarum C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + geranylneryl diphosphate cis,trans,trans-geranylneryl diphosphate Lactiplantibacillus plantarum ?
-
 ?
isopentenyl diphosphate + neryl diphosphate
-
 Lactiplantibacillus plantarum C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + neryl diphosphate
-
 Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + neryl diphosphate
-
 Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
additional information involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components Lactiplantibacillus plantarum ?
-
 ?
additional information involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components Bacillus subtilis ?
-
 ?
additional information involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components Micrococcus luteus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 30000, SDS-PAGE Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Micrococcus luteus
7.5
-
 and a second optimum at pH 10.2, that is 40% of the optimum at pH 7.5 Lactiplantibacillus plantarum
8.5
-
-
 Bacillus subtilis
10.2
-
 and a second optimum at pH 7.5 that is higher than the optimum at pH 10.2 Lactiplantibacillus plantarum