Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzymes in Escherichia coli strain JM109 | Micrococcus luteus |
Protein Variants | Comment | Organism |
---|---|---|
F207S | site-directed mutagenesis, reduced activity, and 13fold increased Km for isopentenyl diphosphate compared to the wild-type enzyme | Micrococcus luteus |
F227S | site-directed mutagenesis | Micrococcus luteus |
W210A | site-directed mutagenesis, reduced activity, unaltered Km for both substrates compared to the wild-type enzyme | Micrococcus luteus |
W224A | site-directed mutagenesis, reduced activity, 6fold increased Km for farnesyl diphosphate, and 14fold increased Km for isopentenyl diphosphate compared to the wild-type enzyme | Micrococcus luteus |
Y148F | site-directed mutagenesis, reduced activity, unaltered Km for both substrates compared to the wild-type enzyme | Micrococcus luteus |
Y148S | site-directed mutagenesis, inactive mutant | Micrococcus luteus |
Y71S | site-directed mutagenesis, reduced activity, unaltered Km for both substrates compared to the wild-type enzyme | Micrococcus luteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
farnesyl diphosphate | pH 7.5, 30°C, recombinant mutant W210A | Micrococcus luteus | |
0.0015 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 30°C, W210A mutant | Micrococcus luteus | |
0.0028 | - |
farnesyl diphosphate | pH 7.5, 30°C, recombinant mutant Y148F | Micrococcus luteus | |
0.0028 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 30°C, Y148F mutant | Micrococcus luteus | |
0.0077 | - |
farnesyl diphosphate | pH 7.5, 30°C, recombinant mutant Y71S | Micrococcus luteus | |
0.0077 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 30°C, Y71S mutant | Micrococcus luteus | |
0.0078 | - |
isopentenyl diphosphate | pH 7.5, 30°C, recombinant wild-type enzyme | Micrococcus luteus | |
0.0078 | - |
isopentenyl diphosphate | pH 7.5, 30°C, wild type | Micrococcus luteus | |
0.0083 | - |
farnesyl diphosphate | pH 7.5, 30°C, recombinant wild-type enzyme | Micrococcus luteus | |
0.0083 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 30°C, wild type | Micrococcus luteus | |
0.01213 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 30°C, W224A mutant | Micrococcus luteus | |
0.0175 | - |
isopentenyl diphosphate | pH 7.5, 30°C, W210A mutant | Micrococcus luteus | |
0.018 | - |
isopentenyl diphosphate | pH 7.5, 30°C, recombinant mutant W210A | Micrococcus luteus | |
0.022 | - |
farnesyl diphosphate | pH 7.5, 30°C, recombinant mutant F207S | Micrococcus luteus | |
0.0222 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 30°C, F207S mutant | Micrococcus luteus | |
0.0229 | - |
isopentenyl diphosphate | pH 7.5, 30°C, Y148F mutant | Micrococcus luteus | |
0.023 | - |
isopentenyl diphosphate | pH 7.5, 30°C, recombinant mutant Y148F | Micrococcus luteus | |
0.0254 | - |
isopentenyl diphosphate | pH 7.5, 30°C, Y71S mutant | Micrococcus luteus | |
0.026 | - |
isopentenyl diphosphate | pH 7.5, 30°C, recombinant mutant Y71S | Micrococcus luteus | |
0.0356 | - |
isopentenyl diphosphate | pH 7.5, 30°C, W224A mutant | Micrococcus luteus | |
0.036 | - |
isopentenyl diphosphate | pH 7.5, 30°C, recombinant mutant Y148F | Micrococcus luteus | |
0.103 | - |
isopentenyl diphosphate | pH 7.5, 30°C, recombinant mutant F207S | Micrococcus luteus | |
0.1031 | - |
isopentenyl diphosphate | pH 7.5, 30°C, F207S mutant | Micrococcus luteus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Micrococcus luteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | Micrococcus luteus | catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | Micrococcus luteus B-P 26 | catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
farnesyl diphosphate + isopentenyl diphosphate | Micrococcus luteus | sequential condensation of 8 molecules of isopentenyl diphosphate in the cis-configuration into farnesyl diphosphate to produce undecaprenyl diphosphate, indispensable for the biosynthesis of bacterial cell wall | diphosphate + di-trans-poly-cis-undecaprenyl diphosphate | - |
? | |
farnesyl diphosphate + isopentenyl diphosphate | Micrococcus luteus B-P 26 | sequential condensation of 8 molecules of isopentenyl diphosphate in the cis-configuration into farnesyl diphosphate to produce undecaprenyl diphosphate, indispensable for the biosynthesis of bacterial cell wall | diphosphate + di-trans-poly-cis-undecaprenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | - |
- |
- |
Micrococcus luteus | O82827 | B-P 26 | - |
Micrococcus luteus B-P 26 | - |
- |
- |
Micrococcus luteus B-P 26 | O82827 | B-P 26 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Micrococcus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Micrococcus luteus | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall | Micrococcus luteus | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Micrococcus luteus B-P 26 | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall | Micrococcus luteus B-P 26 | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
di-trans-poly-cis-decaprenyl diphosphate + isopentenyl diphosphate | di-trans-poly-cis-decaprenyl diphosphate is farnesyl diphosphate, isopentenyl diphosphate binding mode determination | Micrococcus luteus | diphosphate + di-trans-poly-cis-undecaprenyl diphosphate | - |
? | |
di-trans-poly-cis-decaprenyl diphosphate + isopentenyl diphosphate | di-trans-poly-cis-decaprenyl diphosphate is farnesyl diphosphate, isopentenyl diphosphate binding mode determination | Micrococcus luteus B-P 26 | diphosphate + di-trans-poly-cis-undecaprenyl diphosphate | - |
? | |
farnesyl diphosphate + isopentenyl diphosphate | sequential condensation of 8 molecules of isopentenyl diphosphate in the cis-configuration into farnesyl diphosphate to produce undecaprenyl diphosphate, indispensable for the biosynthesis of bacterial cell wall | Micrococcus luteus | diphosphate + di-trans-poly-cis-undecaprenyl diphosphate | - |
? | |
farnesyl diphosphate + isopentenyl diphosphate | sequential condensation of 8 molecules of isopentenyl diphosphate in the cis-configuration into farnesyl diphosphate to produce undecaprenyl diphosphate, indispensable for the biosynthesis of bacterial cell wall | Micrococcus luteus B-P 26 | diphosphate + di-trans-poly-cis-undecaprenyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | cis-type prenyl transferase | Micrococcus luteus |
undecaprenyl diphosphate synthase | - |
Micrococcus luteus |
UPP synthase | - |
Micrococcus luteus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Micrococcus luteus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover number for recombinant wild-type enzyme and mutant enzymes Y71S, Y148F, F207S, W210A, W224A | Micrococcus luteus | |
0.002 | - |
isopentenyl diphosphate | pH 7.5, 30°C, recombinant mutant W210A | Micrococcus luteus | |
0.002 | - |
farnesyl diphosphate | pH 7.5, 30°C, recombinant mutant W210A | Micrococcus luteus | |
0.003 | - |
farnesyl diphosphate | pH 7.5, 30°C, recombinant mutant F207S | Micrococcus luteus | |
0.01 | - |
isopentenyl diphosphate | pH 7.5, 30°C, recombinant mutant F207S | Micrococcus luteus | |
0.015 | - |
farnesyl diphosphate | pH 7.5, 30°C, recombinant mutant Y148F | Micrococcus luteus | |
0.071 | - |
isopentenyl diphosphate | pH 7.5, 30°C, recombinant mutant Y148F | Micrococcus luteus | |
0.071 | - |
farnesyl diphosphate | pH 7.5, 30°C, recombinant mutant Y148F | Micrococcus luteus | |
0.11 | - |
isopentenyl diphosphate | pH 7.5, 30°C, recombinant mutant Y71S | Micrococcus luteus | |
0.11 | - |
farnesyl diphosphate | pH 7.5, 30°C, recombinant mutant Y71S | Micrococcus luteus | |
0.115 | - |
isopentenyl diphosphate | pH 7.5, 30°C, recombinant mutant Y148F | Micrococcus luteus | |
1.22 | - |
farnesyl diphosphate | pH 7.5, 30°C, recombinant wild-type enzyme | Micrococcus luteus | |
1.55 | - |
isopentenyl diphosphate | pH 7.5, 30°C, recombinant wild-type enzyme | Micrococcus luteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Micrococcus luteus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kcat/KM-values for recombinant wild-type enzyme and mutant enzymes Y71S, Y148F, F207S, W210A, W224A | Micrococcus luteus |