Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
using the hanging drop setup | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
DELTAS72 | loop-shortening mutant. Change of the length of the loop 72-83 impairs the ability of conformational change and causes remarkably lower activity of UPPs | Escherichia coli |
DELTAS83 | loop-shortening mutant. Change of the length of the loop 72-83 impairs the ability of conformational change and causes remarkably lower activity of UPPs | Escherichia coli |
DELTAV82S83 | loop-shortening mutant. Change of the length of the loop 72-83 impairs the ability of conformational change and causes remarkably lower activity of UPPs | Escherichia coli |
additional information | loop-shortening mutants with deletion of V82 and S83 or S72 also adopt an open conformation with the loop stretched, although they show decreased intrinsic fluorescence with (2E,6E)-farnesyl diphosphate bound, similar to that seen in the wild-type enzyme | Escherichia coli |
S83(Ala)1 | mutant with amino acids inserted into the loop | Escherichia coli |
S83(Ala)5 | mutant with amino acids inserted into the loop | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C, S83(Ala)5 mutant | Escherichia coli | |
0.0004 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C, wild type | Escherichia coli | |
0.0004 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C, S83(Ala)1 mutant | Escherichia coli | |
0.0005 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C, DELTAS72 mutant | Escherichia coli | |
0.001 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C, DELTAS83 mutant | Escherichia coli | |
0.0018 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C, DELTAV82S83 mutant | Escherichia coli | |
0.0041 | - |
isopentenyl diphosphate | pH 7.5, 25°C, wild type | Escherichia coli | |
0.0078 | - |
isopentenyl diphosphate | pH 7.5, 25°C, S83(Ala)5 mutant | Escherichia coli | |
0.0117 | - |
isopentenyl diphosphate | pH 7.5, 25°C, DELTAS72 mutant | Escherichia coli | |
0.034 | - |
isopentenyl diphosphate | pH 7.5, 25°C, S83(Ala)1 mutant | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Triton | 3 Triton (T1, T2, and T3) are located in the two monomers with one on the top portion of the active site tunnel in monomer A and the other two occupying the overall tunnel of monomer B | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Escherichia coli | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | the two subunits show similar structures | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
undecaprenyl pyrophosphate synthase | - |
Escherichia coli |
UPPs | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00013 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C, S83(Ala)5 mutant | Escherichia coli | |
0.00022 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C, DELTAV82S83 mutant | Escherichia coli | |
0.00028 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C, DELTAS72 mutant | Escherichia coli | |
0.00076 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C, DELTAS83 mutant | Escherichia coli | |
0.0009 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C, S83(Ala)1 mutant | Escherichia coli | |
2.5 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C, wild type | Escherichia coli |