KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
dimethylallyl diphosphate | pH 7.8, 30°C, in presence of 4 mM CaCl2 | Claviceps purpurea | |
0.012 | - |
L-tryptophan | pH 7.8, 30°C, in presence of 4 mM MgCl2 | Claviceps purpurea | |
0.014 | - |
dimethylallyl diphosphate | - |
Claviceps purpurea | |
0.017 | - |
L-tryptophan | pH 7.8, 30°C, in presence of 4 mM CaCl2 | Claviceps purpurea | |
0.04 | - |
L-tryptophan | pH 7.8, 30°C | Claviceps purpurea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | maximal stimulation at 4-10 mM | Claviceps purpurea | |
Mg2+ | maximal stimulation at 4-10 mM | Claviceps purpurea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
alpha2, 2 * 53000, SDS-PAGE | Claviceps purpurea |
105000 | - |
gel filtration | Claviceps purpurea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Claviceps purpurea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Claviceps purpurea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Claviceps purpurea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
- |
Claviceps purpurea |
Storage Stability | Organism |
---|---|
-20°C, glycerol-containing buffer, stable for more than 6 months | Claviceps purpurea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tryptophan | - |
Claviceps purpurea | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha2, 2 * 53000, SDS-PAGE | Claviceps purpurea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Claviceps purpurea |