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Literature summary for 2.5.1.34 extracted from
- Overproduction, purification and characterization of FgaPT2, a dimethylallyltryptophan synthase from Aspergillus fumigatus (2005), Microbiology, 151, 1499-1505.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene fgaPT2, DNA and amino acid sequence determination and analysis, overexpression of His6-tagged enzyme in Saccharomyces cerevisiae INVSc1 | Aspergillus fumigatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
dimethylallyl diphosphate | - |
Aspergillus fumigatus |  |
| 0.008 | - |
L-tryptophan | - |
Aspergillus fumigatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Aspergillus fumigatus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates, but is not essential for activity | Aspergillus fumigatus | |
| Mg2+ | activates, but is not essential for activity | Aspergillus fumigatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52500 | - |
2 * 52500, about, amino acid sequence calculation, 2 * 57000, recombinant His6-tagged enzyme, SDS-PAGE | Aspergillus fumigatus |
| 57000 | - |
2 * 52500, about, amino acid sequence calculation, 2 * 57000, recombinant His6-tagged enzyme, SDS-PAGE | Aspergillus fumigatus |
| 86000 | 91000 | gel filtration | Aspergillus fumigatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + L-tryptophan | Aspergillus fumigatus | first key step in ergot alkaloid biosynthesis providing the pharmaceutically relevant compounds lysergic acid, ergotamine, fumigaclavine A-C, festuclavine, and elymoclavine | diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | Q50EL0 | strain AfA 32C2 and strain B5233, ATCC 13073, gene fgaPT2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme to near homogeneity from Saccharomyces cerevisiae by nickel affinity chromatography | Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + 5-methyl-DL-tryptophan | 11.6% of the activity with L-tryptophan | Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-5-methyl-DL-tryptophan | - |
ir | |
| dimethylallyl diphosphate + 6-methyl-DL-tryptophan | 6.8% of the activity with L-tryptophan | Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-6-methyl-DL-tryptophan | - |
ir | |
| dimethylallyl diphosphate + D-tryptophan | 1.8% of the activity with L-tryptophan | Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-D-tryptophan | - |
ir | |
| dimethylallyl diphosphate + L-tryptophan | first key step in ergot alkaloid biosynthesis providing the pharmaceutically relevant compounds lysergic acid, ergotamine, fumigaclavine A-C, festuclavine, and elymoclavine | Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan | - |
ir | |
| dimethylallyl diphosphate + L-tryptophan | enzyme shows high substrate specificity for dimethylallyl diphosphate and L-tryptophan | Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan | - |
ir | |
| geranyl diphosphate + L-tryptophan | very low activity, 0.7% of the activity with dimethylallyl diphosphate | Aspergillus fumigatus | diphosphate + ? | - |
ir | |
| isopentenyl diphosphate + L-tryptophan | 3.4% of the activity with dimethylallyl diphosphate | Aspergillus fumigatus | diphosphate + ? | - |
ir | |
| additional information | no activity with 4-hydroxyphenylpyruvate, umbelliferone, or 4-hydroxybenzoate the enzyme contains no prenyl diphosphate binding site (N/D)DXXD | Aspergillus fumigatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 52500, about, amino acid sequence calculation, 2 * 57000, recombinant His6-tagged enzyme, SDS-PAGE | Aspergillus fumigatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dimethylallyltryptophan synthase | - |
Aspergillus fumigatus |
| FgaPT2 | - |
Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aspergillus fumigatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Aspergillus fumigatus |
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