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Literature summary for 2.5.1.34 extracted from

  • Luk, L.Y.; Tanner, M.E.
    Mechanism of dimethylallyltryptophan synthase: evidence for a dimethylallyl cation intermediate in an aromatic prenyltransferase reaction (2009), J. Am. Chem. Soc., 131, 13932-13933.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli as a C-terminal His-tagged fusion protein Aspergillus fumigatus

Inhibitors

Inhibitors Comment Organism Structure
EDTA 3 mM EDTA leads to a 2fold reduction Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 dimethylallyl diphosphate
-
 Aspergillus fumigatus
0.011
-
 L-tryptophan
-
 Aspergillus fumigatus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require exogenous divalent metal ions for activity Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
dimethylallyl diphosphate + L-tryptophan = diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan studies are presented that strongly support the dissociative mechanism for the DMAT synthase reaction. The observation of positional isotope exchange (PIX) in isotopically labeled DMAPP provides evidence for the existence of a dimethylallyl carbocation intermediate Aspergillus fumigatus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + L-tryptophan
-
 Aspergillus fumigatus diphosphate + 4-dimethylallyl-L-tryptophan
-
 ?

Synonyms

Synonyms Comment Organism
dimethylallyltryptophan synthase
-
 Aspergillus fumigatus
DMAT synthase
-
 Aspergillus fumigatus