Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein | Aspergillus fumigatus |
Crystallization (Comment) | Organism |
---|---|
x-ray structure of DMATS, determined at a resolution of 1.76 A is reported. A complex of DMATS with its substrate L-tryptophan and with an analogue of its isoprenoid substrate dimethylallyl diphosphate reveals the structural basis of this enzyme-catalyzed Friedel-Crafts reaction, showing strict regiospecificity for position 4 of the indole nucleus of tryptophan as well as unusual independence of the presence of Mg2+ ions. The 3D structure of DMATS belongs to a rare alpha/beta barrel fold, called prenyltransferase barrel, that is present in a small group of bacterial enzymes with no sequence similarity to DMATS | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl S-thiolodiphosphate + L-tryptophan | dimethylallyl S-thiolodiphosphate serves as an analogue to the natural substrate, dimethylallyl diphosphate | Aspergillus fumigatus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dimethylallyltryptophan synthase | - |
Aspergillus fumigatus |
DMATS | - |
Aspergillus fumigatus |