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Literature summary for 2.5.1.44 extracted from
- Homospermidine synthase of Rhodopseudomonas viridis: substrate specificity and effects of the heterologously expressed enzyme of polyamine metabolism of Escherichia coli (1996), J. Gen. Appl. Microbiol., 42, 411-420.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
NAD+ | - |
Blastochloris viridis |  |
| 0.27 | - |
putrescine | - |
Blastochloris viridis | |
| 1.5 | - |
spermidine | in presence of 1 mM putrescine | Blastochloris viridis | |
| 1.7 | - |
putrescine | - |
Blastochloris viridis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activates, optimal concentration at 50 mM | Blastochloris viridis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 putrescine | Blastochloris viridis | - |
sym-homospermidine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Blastochloris viridis | - |
expressed in Escherichia coli strain BL21/pHsRvT72.2 | - |
| Blastochloris viridis | - |
DSM 134 | - |
| Blastochloris viridis | - |
accession no. L77975 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Blastochloris viridis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 putrescine | - |
Blastochloris viridis | sym-homospermidine + NH3 | - |
? | |
| putrescine + 1,3-diaminopropane | - |
Blastochloris viridis | homospermidine + spermidine | - |
? | |
| putrescine + 1,6 diaminohexane | - |
Blastochloris viridis | homospermidine + N-(4-aminobutyl)-1,6-diaminohexane | - |
? | |
| putrescine + 1,7-diaminoheptane | - |
Blastochloris viridis | homospermidine + N-(4-aminobutyl)-1,7-diaminoheptane | - |
? | |
| putrescine + cadaverine | - |
Blastochloris viridis | homospermidine + N-(4-aminobutyl)-1,5-diaminopentane | - |
? | |
| spermidine | - |
Blastochloris viridis | homospermidine + putrescine + 1,3-diaminopropane | - |
? | |
| spermidine + 1,6-diaminohexane | - |
Blastochloris viridis | homospermidine + N-(4-aminobutyl)-1,6-diaminohexane | - |
? | |
| spermidine + cadaverine | - |
Blastochloris viridis | homospermidine + N-(4-aminobutyl)-1,5-diaminopentane | - |
? | |
| spermidine + putrescine | - |
Blastochloris viridis | homospermidine + 1,3-diaminopropane | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
increasing activity up to 45°C, sharp decrease at higher temperatures | Blastochloris viridis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| -18 | - |
storage as acetone dry-powder, stable for several months | Blastochloris viridis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | 9 | in 50 mM potassium phosphate or Bis-Tris-propane buffers | Blastochloris viridis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Blastochloris viridis | |
| NADP+ | 68% of the activity with NAD+ | Blastochloris viridis | |
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Release 2023.1 (January 2023)
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