Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyridoxal | - |
Saccharomyces cerevisiae | |
pyridoxal hydrochloride | 54% inhibition at 1 mM | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-Ser + sulfide | Saccharomyces cerevisiae | functions as a Cys synthase rather than as a homocysteine synthase in vivo | L-Cys + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate | mechanism similar to those of other pyridoxal enzymes | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25.2 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20 C, potassium phosphate buffer, pH 6.5, 1 mM EDTA, at least several days, no loss of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-Ser + sulfide | functions as a Cys synthase rather than as a homocysteine synthase in vivo | Saccharomyces cerevisiae | L-Cys + acetate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
pyridoxal 5'-phosphate stabilizes against heat inactivation | Saccharomyces cerevisiae |
55 | - |
5 min, 45% loss of activity, without pyridoxal 5'-phosphate | Saccharomyces cerevisiae |
65 | - |
5 min, complete loss of activity, without pyridoxal 5'-phosphate | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | stoichiometry, binds to Lys, protects against inactivation by heat, urea, and trypsin, four binding sites per mol apoenzyme, association constant | Saccharomyces cerevisiae |