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Literature summary for 2.5.1.47 extracted from

  • Yamaguchi, T.; Masada, M.
    Comparative studies on cysteine synthase isozymes from spinach leaves (1995), Biochim. Biophys. Acta, 1251, 91-98.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine complete inhibition at 10 mM, isoenzymes 1 and 2, 90% inhibition at 10 mM, isoenzyme 3 Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
H2S pH 8.0, 25°C, isoenzyme 1 Spinacia oleracea
1.25
-
H2S pH 8.0, 25°C, isoenzyme 2 Spinacia oleracea
2.5
-
H2S pH 8.0, 25°C, isoenzyme 3 Spinacia oleracea
3.57
-
O-acetyl-L-Ser pH 8.0, 25°C, isoenzyme 1 Spinacia oleracea
5.26
-
O-acetyl-L-Ser pH 8.0, 25°C, isoenzyme 3 Spinacia oleracea
5.56
-
O-acetyl-L-Ser pH 8.0, 25°C, isoenzyme 2 Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Spinacia oleracea 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
2 * 34000, SDS-PAGE Spinacia oleracea
63000
-
all isoenzymes, gel filtration Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
three isoenzymes
-

Purification (Commentary)

Purification (Comment) Organism
-
Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
400
-
isoenzyme 1 Spinacia oleracea
1400
-
isoenzyme 2 Spinacia oleracea
1431
-
isoenzyme 3 Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-serine
-
Spinacia oleracea L-cysteine + acetate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Spinacia oleracea
More amino acid composition Spinacia oleracea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
2 min, all isoenzymes stable Spinacia oleracea
65
-
2 min, isoenzyme 1 loses 50% loss of activity, isoenzymes 2 loses 25% of its activity, no loss of activity, isoenzyme 3 Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
broad optimum around Spinacia oleracea