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Literature summary for 2.5.1.47 extracted from

  • Mizuno, Y.; Miyashita, Y.; Yamagata, S.; Iwama, T.; Akamatsu, T.
    Cysteine synthase of an extremely thermophilic bacterium, Thermus thermophilus HB8 (2002), Biosci. Biotechnol. Biochem., 66, 549-557.
    View publication on PubMed
Search for more literature for 2.5.1.47

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) 1 mM, 92% inactivation Thermus thermophilus
hydroxylamine 10 mM, 71.2% inhibition Thermus thermophilus
KCN 15.6% inhibition by 1 mM Thermus thermophilus
Monoiodoacetic acid 1 mM, complete inactivation Thermus thermophilus
phenylhydrazine 73% inhibition by 1 mM, 97.4% inhibition by 10 mM Thermus thermophilus
Semicarbazide 13% inhibition by 1 mM, 38.6% inhibition by 10 mM Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 H2S pH 7.8, 50°C Thermus thermophilus
4.8
-
 O-acetyl-L-Ser pH 7.8, 50°C Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 2 * 34000, SDS-PAGE Thermus thermophilus
68000
-
 gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
 HB8
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 HB8
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
337.4
-
-
 Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chloroalanine + sulfide beta-chloroalanine, 6% of the activity with O-acetyl-L-Ser Thermus thermophilus cysteine + chloride
-
 r
chloroalanine + sulfide beta-chloroalanine, 6% of the activity with O-acetyl-L-Ser Thermus thermophilus HB8 / ATCC 27634 / DSM 579 cysteine + chloride
-
 r
L-homoserine + sulfide 1.6% of the activity with O-acetyl-L-serine Thermus thermophilus ?
-
 ?
O-acetyl-L-Ser + H2S
-
 Thermus thermophilus L-Cys + acetate
-
 ?
O-acetyl-L-Ser + H2S
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 L-Cys + acetate
-
 ?
O-acetyl-L-serine
-
 Thermus thermophilus L-cysteine + acetate
-
 ?
O-acetylhomoserine + H2S 2.4% of the activity with O-acetyl-L-Ser Thermus thermophilus homocysteine + ?
-
 ?
O-acetylhomoserine + H2S 2.4% of the activity with O-acetyl-L-Ser Thermus thermophilus HB8 / ATCC 27634 / DSM 579 homocysteine + ?
-
 ?
O-diazoacetyl-L-serine + sulfide 44% of the activity with O-acetyl-Ser Thermus thermophilus ?
-
 ?
O-diazoacetyl-L-serine + sulfide 44% of the activity with O-acetyl-Ser Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
O-succinyl-L-homoserine + sulfide 3.6% of the activity with O-acetyl-L-serine Thermus thermophilus ?
-
 ?
O-succinyl-L-homoserine + sulfide 3.6% of the activity with O-acetyl-L-serine Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
Ser + sulfide 1.8% of the activity with O-acetyl-L-serine Thermus thermophilus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 67 about 50% of maximal activity at 35°C and at 67°C Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 pH 7.8, 60 min, stable Thermus thermophilus
80
-
 60 min, 10 mM dithiothreitol, less than 10% of activity Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermus thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 about 50% of maximal activity at pH 5.5 and pH 8.0 Thermus thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 12 50°C, 30 min, stable Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km of the cofactor at pH 8.0: 0.029 mM Thermus thermophilus