Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.47 extracted from

  • Bermudez, M.A.; Paez-Ochoa, M.A.; Gotor, C.; Romero, L.C.
    Arabidopsis S-sulfocysteine synthase activity is essential for chloroplast function and long-day light-dependent redox control (2010), Plant Cell, 22, 403-416.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.47

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Aminooxyacetate 10 mM, 76% inhibition Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
 O-acetyl-L-serine
-
 Arabidopsis thaliana
0.93
-
 thiosulfate
-
 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-serine + thiosulfate
-
 Arabidopsis thaliana S-sulfo-L-cysteine + acetate
-
 ?

Synonyms

Synonyms Comment Organism
CS26
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
8 9
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction knockout mutants demonstrate a reduction in size and show paleness, but penetrance of the growth phenotype depend on the light regime. The cs26 mutant plants also show reductions in chlorophyll content and photosynthetic activity as well as elevated glutathione levels. cs26 mutant leaves are not able to properly detoxify reactive oxygen species, which accumulate to high levels under long-day growth conditions. The transcriptional profile of the cs26 mutant reveal that the mutation has a pleiotropic effect on many cellular and metabolic processes Arabidopsis thaliana