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Literature summary for 2.5.1.47 extracted from

  • Uda, N.; Matoba, Y.; Kumagai, T.; Oda, K.; Noda, M.; Sugiyama, M
    . Establishment of an in vitro D-cycloserine-synthesizing system by using O-ureido-L-serine synthase and D-cycloserine synthetase found in the biosynthetic pathway (2013), Antimicrob. Agents Chemother., 57, 2603-2612.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.47

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces lavendulae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 hydrogen sulfide 30°C, pH 8.0 Streptomyces lavendulae
22
-
 thiosulfate 30°C, pH 8.0 Streptomyces lavendulae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 4 * 36000, SDS-PAGE Streptomyces lavendulae
126000
-
 gel filtration Streptomyces lavendulae

Organism

Organism UniProt Comment Textmining
Streptomyces lavendulae D2Z027
-
-
Streptomyces lavendulae ATCC 11924 D2Z027
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces lavendulae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-serine + hydrogen sulfide the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate. The enzyme catalyzes the reaction with O-acetyl-L-serine and hydroxyurea with 80fold lower catalytic efficiency Streptomyces lavendulae L-cysteine + acetate
-
 ?
O-acetyl-L-serine + hydrogen sulfide the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate. The enzyme catalyzes the reaction with O-acetyl-L-serine and hydroxyurea with 80fold lower catalytic efficiency Streptomyces lavendulae ATCC 11924 L-cysteine + acetate
-
 ?
O-acetyl-L-serine + L-homocysteine the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate Streptomyces lavendulae cystathionine + acetate
-
 ?
O-acetyl-L-serine + L-homocysteine the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate Streptomyces lavendulae ATCC 11924 cystathionine + acetate
-
 ?
O-acetyl-L-serine + thiosulfate the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate Streptomyces lavendulae S-sulfo-L-cysteine + acetate
-
 ?
O-acetyl-L-serine + thiosulfate the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate Streptomyces lavendulae ATCC 11924 S-sulfo-L-cysteine + acetate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 36000, SDS-PAGE Streptomyces lavendulae

Synonyms

Synonyms Comment Organism
DcsD
-
 Streptomyces lavendulae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces lavendulae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.72
-
 thiosulfate 30°C, pH 8.0 Streptomyces lavendulae
12
-
 hydrogen sulfide 30°C, pH 8.0 Streptomyces lavendulae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Streptomyces lavendulae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.033
-
 thiosulfate 30°C, pH 8.0 Streptomyces lavendulae
0.14
-
 O-acetyl-L-serine 30°C, pH 8.0, cosubstrate: hydrogen sulfide Streptomyces lavendulae
0.19
-
 L-homocysteine 30°C, pH 8.0 Streptomyces lavendulae
100
-
 hydrogen sulfide 30°C, pH 8.0 Streptomyces lavendulae