Protein Variants | Comment | Organism |
---|---|---|
D45A | decrease in kcat and increase in Km value of succinyl-L-homoserine substrate | Escherichia coli |
D45N | decrease in kcat and increase in Km value of succinyl-L-homoserine substrate | Escherichia coli |
E325A | decrease in kcat and increase in Km values of substrates | Escherichia coli |
E325Q | kinetic values similar to wild-type | Escherichia coli |
N227A | decrease in kcat value of succinyl-L-homoserine substrate | Escherichia coli |
R106A | the Arg residues at positions 48, 106 and 361 tether the distal and alpha-carboxylate moieties, respectively, of the succinyl-L-homoserine substrate | Escherichia coli |
R106K | the Arg residues at positions 48, 106 and 361 tether the distal and alpha-carboxylate moieties, respectively, of the succinyl-L-homoserine substrate | Escherichia coli |
R361A | almost complete loss of activity | Escherichia coli |
R361K | the Arg residues at positions 48, 106 and 361 tether the distal and alpha-carboxylate moieties, respectively, of the succinyl-L-homoserine substrate | Escherichia coli |
R48A | almost complete loss of activity | Escherichia coli |
R48K | the Arg residues at positions 48, 106 and 361 tether the distal and alpha-carboxylate moieties, respectively, of the succinyl-L-homoserine substrate | Escherichia coli |
R49A | decrease in kcat value | Escherichia coli |
R49K | 3fold increase in Km value for both succinyl-L-homoserine and L-cysteine | Escherichia coli |
S326A | decrease in kcat and increase in Km value of succinyl-L-homoserine substrate | Escherichia coli |
Y101F | decrease in kcat value | Escherichia coli |
Y46F | large decrease in kcat and increase in Km value of succinyl-L-homoserine substrate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-cysteine | mutant R49A, pH 7.8, 25°C | Escherichia coli | |
0.15 | - |
L-cysteine | mutant Y101F, pH 7.8, 25°C | Escherichia coli | |
0.17 | - |
L-cysteine | mutant E325A, pH 7.8, 25°C | Escherichia coli | |
0.2 | - |
L-cysteine | mutant S326A, pH 7.8, 25°C | Escherichia coli | |
0.22 | - |
L-cysteine | mutant R361K, pH 7.8, 25°C | Escherichia coli | |
0.22 | - |
L-cysteine | mutant R48K, pH 7.8, 25°C | Escherichia coli | |
0.24 | - |
L-cysteine | wild-type, pH 7.8, 25°C | Escherichia coli | |
0.29 | - |
L-cysteine | mutant E325Q, pH 7.8, 25°C | Escherichia coli | |
0.3 | - |
L-cysteine | mutant N227A, pH 7.8, 25°C | Escherichia coli | |
0.36 | - |
L-cysteine | mutant R49K, pH 7.8, 25°C | Escherichia coli | |
0.6 | - |
L-cysteine | mutant Y46F, pH 7.8, 25°C | Escherichia coli | |
0.65 | - |
L-cysteine | mutant R106K, pH 7.8, 25°C | Escherichia coli | |
0.7 | - |
O-succinyl-L-homoserine | mutant E325A, pH 7.8, 25°C | Escherichia coli | |
0.8 | - |
L-cysteine | mutant D45A, pH 7.8, 25°C | Escherichia coli | |
0.9 | - |
L-cysteine | mutant D45N, pH 7.8, 25°C | Escherichia coli | |
1.1 | - |
O-succinyl-L-homoserine | mutant E325Q, pH 7.8, 25°C | Escherichia coli | |
1.8 | - |
O-succinyl-L-homoserine | mutant R48K, pH 7.8, 25°C | Escherichia coli | |
1.9 | - |
O-succinyl-L-homoserine | mutant Y101F, pH 7.8, 25°C | Escherichia coli | |
3 | - |
L-cysteine | mutant R106A, pH 7.8, 25°C | Escherichia coli | |
4.4 | - |
O-succinyl-L-homoserine | wild-type, pH 7.8, 25°C | Escherichia coli | |
8 | - |
O-succinyl-L-homoserine | mutant N227A, pH 7.8, 25°C | Escherichia coli | |
8 | - |
O-succinyl-L-homoserine | mutant R49K, pH 7.8, 25°C | Escherichia coli | |
11 | - |
O-succinyl-L-homoserine | mutant R106A, pH 7.8, 25°C | Escherichia coli | |
19 | - |
O-succinyl-L-homoserine | mutant D45A, pH 7.8, 25°C | Escherichia coli | |
21 | - |
O-succinyl-L-homoserine | mutant S326A, pH 7.8, 25°C | Escherichia coli | |
39 | - |
O-succinyl-L-homoserine | mutant D45N, pH 7.8, 25°C | Escherichia coli | |
39 | - |
O-succinyl-L-homoserine | mutant R106K, pH 7.8, 25°C | Escherichia coli | |
90 | - |
O-succinyl-L-homoserine | mutant Y46F, pH 7.8, 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-succinyl-L-homoserine + L-cysteine | - |
Escherichia coli | L-cystathionine + succinate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
O-succinyl-L-homoserine | mutant R48K, pH 7.8, 25°C | Escherichia coli | |
0.31 | - |
O-succinyl-L-homoserine | mutant S326A, pH 7.8, 25°C | Escherichia coli | |
0.5 | - |
O-succinyl-L-homoserine | mutant R361K, pH 7.8, 25°C | Escherichia coli | |
1.4 | - |
O-succinyl-L-homoserine | mutant R106A, pH 7.8, 25°C | Escherichia coli | |
5.3 | - |
O-succinyl-L-homoserine | mutant R106K, pH 7.8, 25°C | Escherichia coli | |
5.6 | - |
O-succinyl-L-homoserine | mutant R49A, pH 7.8, 25°C | Escherichia coli | |
6 | - |
O-succinyl-L-homoserine | mutant Y46F, pH 7.8, 25°C | Escherichia coli | |
9.6 | - |
O-succinyl-L-homoserine | mutant Y101F, pH 7.8, 25°C | Escherichia coli | |
33 | - |
O-succinyl-L-homoserine | mutant N227A, pH 7.8, 25°C | Escherichia coli | |
57 | - |
O-succinyl-L-homoserine | mutant E325Q, pH 7.8, 25°C | Escherichia coli | |
59 | - |
O-succinyl-L-homoserine | mutant E325A, pH 7.8, 25°C | Escherichia coli | |
79 | - |
O-succinyl-L-homoserine | mutant D45A, pH 7.8, 25°C | Escherichia coli | |
100 | - |
O-succinyl-L-homoserine | mutant D45N, pH 7.8, 25°C | Escherichia coli | |
112 | - |
O-succinyl-L-homoserine | wild-type, pH 7.8, 25°C | Escherichia coli | |
130 | - |
O-succinyl-L-homoserine | mutant R49K, pH 7.8, 25°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
O-succinyl-L-homoserine | mutant R48K, pH 7.8, 25°C | Escherichia coli | |
0.005 | - |
O-succinyl-L-homoserine | mutant R361K, pH 7.8, 25°C | Escherichia coli | |
0.015 | - |
O-succinyl-L-homoserine | mutant S326A, pH 7.8, 25°C | Escherichia coli | |
0.071 | - |
O-succinyl-L-homoserine | mutant Y46F, pH 7.8, 25°C | Escherichia coli | |
0.12 | - |
O-succinyl-L-homoserine | mutant R106A, pH 7.8, 25°C | Escherichia coli | |
0.14 | - |
O-succinyl-L-homoserine | mutant R106K, pH 7.8, 25°C | Escherichia coli | |
0.41 | - |
L-cysteine | mutant R106A, pH 7.8, 25°C | Escherichia coli | |
0.68 | - |
L-cysteine | mutant R48K, pH 7.8, 25°C | Escherichia coli | |
1.6 | - |
L-cysteine | mutant S326A, pH 7.8, 25°C | Escherichia coli | |
2.3 | - |
L-cysteine | mutant R361K, pH 7.8, 25°C | Escherichia coli | |
3.1 | - |
O-succinyl-L-homoserine | mutant R49A, pH 7.8, 25°C | Escherichia coli | |
4.2 | - |
O-succinyl-L-homoserine | mutant N227A, pH 7.8, 25°C | Escherichia coli | |
5.1 | - |
O-succinyl-L-homoserine | mutant Y101F, pH 7.8, 25°C | Escherichia coli | |
8.2 | - |
L-cysteine | mutant R106K, pH 7.8, 25°C | Escherichia coli | |
11.3 | - |
L-cysteine | mutant Y46F, pH 7.8, 25°C | Escherichia coli | |
16 | - |
O-succinyl-L-homoserine | mutant R49K, pH 7.8, 25°C | Escherichia coli | |
25 | - |
O-succinyl-L-homoserine | wild-type, pH 7.8, 25°C | Escherichia coli | |
26 | - |
O-succinyl-L-homoserine | mutant D45N, pH 7.8, 25°C | Escherichia coli | |
42 | - |
O-succinyl-L-homoserine | mutant D45A, pH 7.8, 25°C | Escherichia coli | |
50 | - |
O-succinyl-L-homoserine | mutant E325Q, pH 7.8, 25°C | Escherichia coli | |
50 | - |
L-cysteine | mutant R49A, pH 7.8, 25°C | Escherichia coli | |
64 | - |
L-cysteine | mutant Y101F, pH 7.8, 25°C | Escherichia coli | |
90 | - |
O-succinyl-L-homoserine | mutant E325A, pH 7.8, 25°C | Escherichia coli | |
99 | - |
L-cysteine | mutant D45A, pH 7.8, 25°C | Escherichia coli | |
110 | - |
L-cysteine | mutant N227A, pH 7.8, 25°C | Escherichia coli | |
113 | - |
L-cysteine | mutant D45N, pH 7.8, 25°C | Escherichia coli | |
200 | - |
L-cysteine | mutant E325Q, pH 7.8, 25°C | Escherichia coli | |
350 | - |
L-cysteine | mutant E325A, pH 7.8, 25°C | Escherichia coli | |
360 | - |
L-cysteine | mutant R49K, pH 7.8, 25°C | Escherichia coli | |
470 | - |
L-cysteine | wild-type, pH 7.8, 25°C | Escherichia coli |