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Literature summary for 2.5.1.49 extracted from
- O-Alkylhomoserine synthesis catalyzed by O-acetylhomoserine sulfhydrylase in microorganisms (1977), J. Bacteriol., 130, 62-73.
General Stability
| General Stability | Organism |
|---|---|
| not stable even in presence of a cofactor | Corynebacterium acetophilum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| butanol | - |
Corynebacterium acetophilum |  |
| hydrazine | - |
Corynebacterium acetophilum | |
| Hydroxylamine hydrochloride | - |
Corynebacterium acetophilum | |
| Isopropanol | - |
Corynebacterium acetophilum | |
| KCN | - |
Corynebacterium acetophilum | |
| L-homoserine | - |
Corynebacterium acetophilum | |
| methanol | - |
Corynebacterium acetophilum | |
| methylmercaptan | - |
Corynebacterium acetophilum | |
| n-Propanol | - |
Corynebacterium acetophilum | |
| Na2S | - |
Corynebacterium acetophilum | |
| O-acetyl-L-serine | - |
Corynebacterium acetophilum | |
| O-acetyl-L-threonine | - |
Corynebacterium acetophilum | |
| O-ethylhomoserine | - |
Corynebacterium acetophilum | |
| O-succinyl-DL-homoserine | - |
Corynebacterium acetophilum | |
| p-chloromercuribenzoate | - |
Corynebacterium acetophilum | |
| phenylhydrazine | - |
Corynebacterium acetophilum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
O-acetyl-L-homoserine | O-alkylhomoserine synthesis | Corynebacterium acetophilum | |
| 2.4 | - |
H2S | - |
Corynebacterium acetophilum | |
| 13 | - |
O-acetyl-L-homoserine | O-acetylhomoserine sulfhydrylation | Corynebacterium acetophilum | |
| 40 | - |
ethanol | - |
Corynebacterium acetophilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
2 * 110000, SDS-PAGE | Corynebacterium acetophilum |
| 220000 | - |
gel filtration | Corynebacterium acetophilum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-homoserine + H2S | Corynebacterium acetophilum | - |
L-homocysteine + acetic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium acetophilum | - |
- |
- |
| Corynebacterium acetophilum A51 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Corynebacterium acetophilum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.108 | - |
O-alkylhomoserine synthesizing activity | Corynebacterium acetophilum |
| 16.7 | - |
O-acetylhomoserine sulfhydrylase activity | Corynebacterium acetophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | O-acetyl-L-serine, L-homoserine, O-succinyl-L-homoserine and O-acetyl-L-threonine are no substrates | Corynebacterium acetophilum | ? | - |
? | |
| additional information | O-acetyl-L-serine, L-homoserine, O-succinyl-L-homoserine and O-acetyl-L-threonine are no substrates | Corynebacterium acetophilum A51 | ? | - |
? | |
| O-acetyl-L-homoserine + ethanol | - |
Corynebacterium acetophilum | O-ethyl-homoserine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + ethanol | - |
Corynebacterium acetophilum A51 | O-ethyl-homoserine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + H2S | - |
Corynebacterium acetophilum | L-homocysteine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + methanol | - |
Corynebacterium acetophilum | O-methyl-homoserine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + methyl mercaptan | - |
Corynebacterium acetophilum | L-methionine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + methyl mercaptan | - |
Corynebacterium acetophilum A51 | L-methionine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + n-butanol | - |
Corynebacterium acetophilum | O-butyl-homoserine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + n-butanol | - |
Corynebacterium acetophilum A51 | O-butyl-homoserine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + n-pentanol | - |
Corynebacterium acetophilum | O-pentyl-homoserine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + n-propanol | - |
Corynebacterium acetophilum | O-propyl-homoserine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + n-propanol | - |
Corynebacterium acetophilum A51 | O-propyl-homoserine + acetic acid | - |
? | |
| O-acetyl-L-serine + H2S | - |
Corynebacterium acetophilum | L-cysteine + acetic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 110000, SDS-PAGE | Corynebacterium acetophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | 45 | - |
Corynebacterium acetophilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Corynebacterium acetophilum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | tightly bound | Corynebacterium acetophilum | |
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