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Literature summary for 2.5.1.49 extracted from

  • Yamagata, S.; Isaji, M.; Nakamura, K.; Fujisaki, S.; Doi, K.; Bawden, S.; D'Andrea, R.
    Overexpression of the Saccharomyces cerevisiae MET17/MET25 gene in Escherichia coli and comparative characterization of the product with O-acetylserine-O-acetylhomoserine sulfhydrylase of the yeast (1994), Appl. Microbiol. Biotechnol., 42, 92-99.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
MET17/MET25 gene cloned and overexpressed in Escherichia coli Saccharomyces cerevisiae

General Stability

General Stability Organism
sensitive to trypsin digestion Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Hydroxylamine hydrochloride
-
 Saccharomyces cerevisiae
L-Penicillamine
-
 Saccharomyces cerevisiae
phenylhydrazine
-
 Saccharomyces cerevisiae
Semicarbazide
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.7
-
 O-acetyl-L-homoserine wild-type enzyme Saccharomyces cerevisiae
9
-
 O-acetyl-L-homoserine recombinant enzyme Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and recombinant enzyme Saccharomyces cerevisiae