Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.49 extracted from

  • Shimizu, H.; Yamagata, S.; Masui, R.; Inoue,Y.; Shibata, T.; Yokoyama, S.; Kuramitsu, S.; Iwama, T.
    Cloning and overexpression of the oah1 gene encoding O-acetyl-L-homoserine sulfhydrylase of Thermus thermophilus HB8 and characterization of the gene product (2001), Biochim. Biophys. Acta, 1549, 61-72.
    View publication on PubMed
Search for more literature for 2.5.1.49

Cloned(Commentary)

Cloned (Comment) Organism
oah1 gene cloned, sequenced and overexpressed in Escherichia coli BL21(DE3) Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
DL-C-propagylglycine
-
 Thermus thermophilus
Hydroxylamine hydrochloride
-
 Thermus thermophilus
L-methionine
-
 Thermus thermophilus
phenylhydrazine
-
 Thermus thermophilus
Semicarbazide
-
 Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 H2S
-
 Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 4 * 46000, calculated from OAH1 sequence Thermus thermophilus
46059
-
 4 * 46059, calculated from amino acid sequence Thermus thermophilus
47000
-
 4 * 47000, SDS-PAGE Thermus thermophilus
163000
-
 gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q93I77 DDBJ: AB049221, EMBL:AB049221
-
Thermus thermophilus Q93I77 nucleotide sequence accession no.
-
Thermus thermophilus Q93I77 HB8, HB27
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q93I77 DDBJ: AB049221, EMBL:AB049221
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
 cysthathione beta-lyase reaction Thermus thermophilus
0.58
-
 L-homoserine as substrate Thermus thermophilus
0.72
-
 O-succinyl-L-homoserine as substrate Thermus thermophilus
1.33
-
 L-serine as substrate Thermus thermophilus
1.57
-
 O-phospho-L-serine as substrate Thermus thermophilus
2.12
-
 O-acetyl-L-serine as substrate Thermus thermophilus
40.7
-
 recombinant enzyme Thermus thermophilus
49.82
-
 O-acetyl-L-homoserine as substrate Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-homoserine + H2S
-
 Thermus thermophilus homocysteine + ?
-
 ?
L-homoserine + H2S
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 homocysteine + ?
-
 ?
additional information low cystathione beta-lyase reaction, relative activity 0.12%, no cystathione gamma-lyase reaction Thermus thermophilus ?
-
 ?
additional information low cystathione beta-lyase reaction, relative activity 0.12%, no cystathione gamma-lyase reaction Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
O-acetyl-L-serine + H2S
-
 Thermus thermophilus L-cysteine + acetic acid
-
 ?
O-acetyl-L-serine + H2S
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 L-cysteine + acetic acid
-
 ?
O-phospho-L-serine + H2S
-
 Thermus thermophilus ?
-
 ?
O-phospho-L-serine + H2S
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
O-succinyl-L-homoserine + H2S
-
 Thermus thermophilus homocysteine + ?
-
 ?
O-succinyl-L-homoserine + H2S
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 homocysteine + ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 47000, SDS-PAGE Thermus thermophilus
tetramer 4 * 46059, calculated from amino acid sequence Thermus thermophilus
tetramer 4 * 46000, calculated from OAH1 sequence Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 85
-
 Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 very stable at high temperatures, 90% activity at 90°C for 60 min at pH 7.8 Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Thermus thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
4 12 50% maximum activity at pH 6.3 and pH 9.3 Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Thermus thermophilus