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Literature summary for 2.5.1.49 extracted from

  • Iwama, T.; Hosokawa, H.; Lin, W.; Shimizu, H.; Kawai, K.; Yamagata, S.
    Comparative characterization of the oah2 gene homologous to the oah1 of Thermus thermophilus HB8 (2004), Biosci. Biotechnol. Biochem., 68, 1357-1361.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
DL-propargylglycine 89% inhibition at 1 mM Thermus thermophilus
hydroxylamine 95% inhibition at 1 mM, 76% at 0.1 mM Thermus thermophilus
L-methionine 68% inhibition at 1 mM Thermus thermophilus
phenylhydrazine 95% inhibition at 10 mM, 12% at 1 mM Thermus thermophilus
Semicarbazide 76% inhibition at 10 mM, 12% at 1 mM Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
O-acetyl-L-homoserine at a fixed sulfide concentration of 1 mM Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
SDS-PAGE Thermus thermophilus
44160
-
calculated from amino acid sequence Thermus thermophilus
190000
-
gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q76K51
-
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q76K51
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.17
-
crude extract Thermus thermophilus
14.3
-
after heat treatment at 65°C Thermus thermophilus
36
-
Q Sepharose pool Thermus thermophilus
67.7
-
phenyl Sepharose pool Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-homoserine + H2S lesser activity Thermus thermophilus L-homocysteine + H2O
-
?
L-homoserine + H2S lesser activity Thermus thermophilus HB8 / ATCC 27634 / DSM 579 L-homocysteine + H2O
-
?
L-serine + H2S lesser activity Thermus thermophilus L-cysteine + H2O
-
?
L-serine + H2S lesser activity Thermus thermophilus HB8 / ATCC 27634 / DSM 579 L-cysteine + H2O
-
?
o-acetyl-L-homoserine + H2S
-
Thermus thermophilus L-homocysteine + acetate
-
?
o-acetyl-L-homoserine + H2S
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 L-homocysteine + acetate
-
?
O-acetyl-L-serine lesser activity Thermus thermophilus L-cysteine + acetate
-
?
O-acetyl-L-serine lesser activity Thermus thermophilus HB8 / ATCC 27634 / DSM 579 L-cysteine + acetate
-
?
O-phosphoryl-L-serine + H2S lesser activity Thermus thermophilus L-cysteine + H2O
-
?
O-succinyl-L-homoserine + H2S lesser activity Thermus thermophilus L-homocysteine + succinate
-
?
O-succinyl-L-homoserine + H2S lesser activity Thermus thermophilus HB8 / ATCC 27634 / DSM 579 L-homocysteine + succinate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 44000, SDS-PAGE Thermus thermophilus
tetramer 4 * 44000, SDS-PAGE, gel filtration Thermus thermophilus

Synonyms

Synonyms Comment Organism
EC 4.2.99.10 formerly Thermus thermophilus
o-acetyl-L-homoserine sulfhydrylase
-
Thermus thermophilus
OAH SHase
-
Thermus thermophilus
oah2 gene product
-
Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
80% activity remaining at 90°C for 60 min Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Thermus thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
6.3 8.8 less than 50% activity above and below Thermus thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 12
-
Thermus thermophilus