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Literature summary for 2.5.1.54 extracted from
- Effect of glyphosate on the activity of DAHP synthase isozymes in callus cultures of groundnut (Arachis hypogaea L.) selected in vitro (2000), Indian J. Biochem. Biophys., 37, 235-240.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Tyr | 2.3fold activation of isozyme DS-Mn | Arachis hypogaea |  |
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | isozyme DS-Co is a possible target for the herbicide glyphosate, i.e. N-[phosphomonomethyl]glycine | Arachis hypogaea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Arachis hypogaea | |
| N-[Phosphomonomethyl]glycine | i.e. glyphosate; inhibition of isozyme DS-Co in vitro and in vivo, isozyme DS-Mn is only slightly inhibited in vitro | Arachis hypogaea | |
| Phe | no inhibition | Arachis hypogaea | |
| Trp | isozyme DS-Mn | Arachis hypogaea | |
| Tyr | no inhibition | Arachis hypogaea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | isozyme DS-Co | Arachis hypogaea | |
| Mn2+ | isozyme DS-Mn | Arachis hypogaea | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arachis hypogaea | - |
2 isozymes: DS-Co and DS-Mn | - |
| Arachis hypogaea | - |
cv. JL-24 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially, isozymes DS-Co and DS-Mn | Arachis hypogaea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | - |
Arachis hypogaea | - |
| leaf | - |
Arachis hypogaea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.11 | - |
partially purified isozyme DS-Mn, with Mn2+ | Arachis hypogaea |
| 0.14 | - |
partially purified isozyme DS-Co, with Co2+ | Arachis hypogaea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Arachis hypogaea | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Arachis hypogaea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Arachis hypogaea |
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