Cloned (Comment) | Organism |
---|---|
expressed in soluble form by co-expression with chaperonins GroEL/GroES in Escherichia coli | Actinosynnema pretiosum subsp. auranticum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 2 mM, complete inactivation within 10 min | Actinosynnema pretiosum subsp. auranticum | |
additional information | substrate D-erythrose 4-phosphate is not inhibitory | Actinosynnema pretiosum subsp. auranticum | |
phosphoenolpyruvate | pronounced substrate inhibition above 1 mM | Actinosynnema pretiosum subsp. auranticum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
phosphoenolpyruvate | pH 7.5, 30°C | Actinosynnema pretiosum subsp. auranticum | |
0.43 | - |
D-erythrose 4-phosphate | pH 7.5, 30°C | Actinosynnema pretiosum subsp. auranticum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.1 mM, up to 500% of initial activity | Actinosynnema pretiosum subsp. auranticum | |
Co2+ | 0.1 mM, up to 535% of initial activity | Actinosynnema pretiosum subsp. auranticum | |
Mn2+ | 0.1 mM, up to 550% of initial activity | Actinosynnema pretiosum subsp. auranticum | |
Ni2+ | 0.1 mM, up to 495% of initial activity | Actinosynnema pretiosum subsp. auranticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinosynnema pretiosum subsp. auranticum | O68903 | - |
- |
Actinosynnema pretiosum subsp. auranticum ATCC 31565 | O68903 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0127 | - |
pH 7.5, 30°C | Actinosynnema pretiosum subsp. auranticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Actinosynnema pretiosum subsp. auranticum | 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Actinosynnema pretiosum subsp. auranticum ATCC 31565 | 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aroF | - |
Actinosynnema pretiosum subsp. auranticum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
D-erythrose 4-phosphate | pH 7.5, 30°C | Actinosynnema pretiosum subsp. auranticum | |
0.032 | - |
phosphoenolpyruvate | pH 7.5, 30°C | Actinosynnema pretiosum subsp. auranticum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Actinosynnema pretiosum subsp. auranticum | predicted | - |
5.5 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.069 | - |
D-erythrose 4-phosphate | pH 7.5, 30°C | Actinosynnema pretiosum subsp. auranticum | |
0.35 | - |
phosphoenolpyruvate | pH 7.5, 30°C | Actinosynnema pretiosum subsp. auranticum |