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Literature summary for 2.5.1.55 extracted from
- Purification and characterization of 3-deoxy-D-manno-octulosonate 8-phosphate synthetase from Escherichia coli (1980), J. Bacteriol., 141, 635-644.
General Stability
| General Stability | Organism |
|---|---|
| dialysis against 10 mM EDTA in 50 mM Tris-HCl buffer, pH 7.3, inactivates | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Escherichia coli |  |
| Cu2+ | - |
Escherichia coli | |
| D-ribose 5-phosphate | weak, competitive | Escherichia coli | |
| Hg2+ | reversed by dithiothreitol | Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
phosphoenolpyruvate | pH 7.3, 37°C | Escherichia coli | |
| 0.02 | - |
D-arabinose 5-phosphate | pH 7.3, 37°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 1 mM, 5-10% stimulation | Escherichia coli | |
| Fe3+ | 1 mM, 5-10% stimulation | Escherichia coli | |
| Li+ | 1 mM, 5-10% stimulation | Escherichia coli | |
| Mg2+ | 1 mM, 5-10% stimulation | Escherichia coli | |
| Mn2+ | 1 mM, 5-10% stimulation | Escherichia coli | |
| additional information | no metal requirement | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
3 * 32000, SDS-PAGE | Escherichia coli |
| 90000 | - |
gel filtration, non-denaturing PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.7 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + D-arabinose 5-phosphate | - |
Escherichia coli | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? | |
| phosphoenolpyruvate + D-arabinose 5-phosphate | - |
Escherichia coli B / ATCC 11303 | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 32000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
15 min, 30% loss of activity | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | and a second optimum at pH 9.0 | Escherichia coli |
| 9 | - |
and a second optimum at pH 4.0-6.0 | Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
D-ribose 5-phosphate | pH 7.3, 37°C | Escherichia coli | |
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