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Literature summary for 2.5.1.55 extracted from

  • Rick, P.D.; Young, D.A.
    Isolation and characterization of a temperature-sensitive lethal mutant of Salmonella thyphimurium that is conditionally defective in 3-deoxy-D-manno-octulosonate-8-phosphate synthesis (1982), J. Bacteriol., 150, 447-455.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.55

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 D-arabinose 5-phosphate 28°C, parent strain PR122 Salmonella enterica subsp. enterica serovar Typhimurium
0.22
-
 D-arabinose 5-phosphate 37°C, parent strain PR122 Salmonella enterica subsp. enterica serovar Typhimurium
0.23
-
 D-arabinose 5-phosphate 37°C, mutant strain PR32 Salmonella enterica subsp. enterica serovar Typhimurium
0.45
-
 D-arabinose 5-phosphate 42°C, mutant strain PR32 Salmonella enterica subsp. enterica serovar Typhimurium
0.83
-
 D-arabinose 5-phosphate 42°C, parent strain PR122 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
 parent strain PR122, and mutant strain PR32, a temperature-sensitive lethal mutant that is conditionally defective in 3-deoxy-D-manno-octulosonate-8-phosphate synthesis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-arabinose 5-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 temperature-sensitive lethal mutant of Salmonella thyphimurium that is conditionally defective in 3-deoxy-D-manno-octulosonate-8-phosphate synthesis due to temperature-sensitive phospho-2-dehydro-3-deoxyoctonate aldolase Salmonella enterica subsp. enterica serovar Typhimurium
37
-
 half-life of enzyme from mutant strain PR32, without D-arabinose-5-phosphate: 4.5 min. Half-life of enzyme from mutant strain PR32, in presence of 13 mM D-arabinose 5-phosphate: 18 min. Half-life of enzyme from parent strain PR122: 31 min Salmonella enterica subsp. enterica serovar Typhimurium
42
-
 half-life of enzyme from mutant strain PR32, without D-arabinose-5-phosphate: 2.5 min. Half-life of enzyme from mutant strain PR32, in presence of 13 mM D-arabinose 5-phosphate: 9.5 min. Half-life of enzyme from parent strain PR122: 13 min Salmonella enterica subsp. enterica serovar Typhimurium