Crystallization (Comment) | Organism |
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- |
Aquifex aeolicus |
Protein Variants | Comment | Organism |
---|---|---|
H185G | mutation decreases the affinity of the enzyme to bind Fe2+, but not Zn2+. Maximal activity, about 8-10% of the wild-type activity is obtained when the native metal is replaced with Cd2+ | Aquifex aeolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | the enzyme is most active when the endogenous metal is removed by incubation with EDTA and replaced with Cd2+ | Aquifex aeolicus | |
Fe2+ | wild-type enzyme contains Zn2+ and Fe2+ with the ratio Zn2+/Fe2+ ranging from 1 to 2 in different preparations. Mutation H185G decreases the ability of the enzyme to bind Fe2+, but not Zn2+. Maximal activity, about 8-10% of the wild-type activity is obtained when the native metal is replaced with Cd2+ | Aquifex aeolicus | |
Zn2+ | wild-type enzyme contains Zn2+ and Fe2+ with the ratio Zn2+/Fe2+ ranging from 1 to 2 in different preparations | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O66496 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-arabinose 5-phosphate | His185 is necessary for the correct binding of phosphoenolpyruvate and of a catalytic water molecule | Aquifex aeolicus | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? |