Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
D-arabinose 5-phosphate | pH 7.5, 60°C | Aquifex aeolicus | |
0.015 | - |
D-arabinose 5-phosphate | pH 7.5, 70°C | Aquifex aeolicus | |
0.027 | - |
D-arabinose 5-phosphate | pH 7.5, 80°C | Aquifex aeolicus | |
0.028 | - |
phosphoenolpyruvate | pH 7.5, at 90°C | Aquifex aeolicus | |
0.038 | - |
phosphoenolpyruvate | pH 7.5, at 80°C | Aquifex aeolicus | |
0.043 | - |
phosphoenolpyruvate | pH 7.5, at 60°C or 70°C | Aquifex aeolicus | |
0.074 | - |
D-arabinose 5-phosphate | pH 7.5, 90°C | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29736 | - |
3 * 29736, electrospray ionization mass spectrometry | Aquifex aeolicus |
97600 | - |
gel filtration | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aquifex aeolicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.38 | - |
- |
Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-arabinose 5-phosphate | - |
Aquifex aeolicus | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 3 * 29736, electrospray ionization mass spectrometry | Aquifex aeolicus |
trimer | - |
Aquifex aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Aquifex aeolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life: 30.3 h | Aquifex aeolicus |
80 | - |
half-life: 8.1 h | Aquifex aeolicus |
90 | - |
half-life: 1.5 h | Aquifex aeolicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
phosphoenolpyruvate | pH 7.5, 60°C | Aquifex aeolicus | |
0.38 | - |
D-arabinose 5-phosphate | pH 7.5, 60°C | Aquifex aeolicus | |
0.87 | - |
phosphoenolpyruvate | pH 7.5, 70°C | Aquifex aeolicus | |
0.87 | - |
D-arabinose 5-phosphate | pH 7.5, 70°C | Aquifex aeolicus | |
1.47 | - |
phosphoenolpyruvate | pH 7.5, 80°C | Aquifex aeolicus | |
1.47 | - |
D-arabinose 5-phosphate | pH 7.5, 80°C | Aquifex aeolicus | |
2 | - |
phosphoenolpyruvate | pH 7.5, 90°C | Aquifex aeolicus | |
2 | - |
D-arabinose 5-phosphate | pH 7.5, 90°C | Aquifex aeolicus |