Literature summary for 2.5.1.55 extracted from

Allison, T.M.; Hutton, R.D.; Cochrane, F.C.; Yeoman, J.A.; Jameson, G.B.; Parker, E.J.
Targeting the role of a key conserved motif for substrate selection and catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase (2011), Biochemistry, 50, 3686-3695.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
the X-ray structures, determined for the mutants with altered KANRS motif, indicate no gross structural penalty resulting from mutation, but the subtle changes observed in the active sites of these mutant proteins correlate with their altered catalytic function	Neisseria meningitidis

Protein Variants

Protein Variants	Comment	Organism
A56P/N57DELTA	mutation in the absolutely conserved KANRS motif. Complete loss of activity	Acidithiobacillus ferrooxidans
A56P/N57DELTA	mutation in the absolutely conserved KANRS motif. Complete loss of activity	Neisseria meningitidis
K55A	mutation in the absolutely conserved KANRS motif. Complete loss of activity	Acidithiobacillus ferrooxidans
K57A	mutation in the absolutely conserved KANRS motif. Complete loss of activity	Neisseria meningitidis
N57A	mutation in the absolutely conserved KANRS motif. About 2% of wild-type activity	Acidithiobacillus ferrooxidans
N57D	mutation in the absolutely conserved KANRS motif. Complete loss of activity	Neisseria meningitidis
N57DELTA	mutation in the absolutely conserved KANRS motif. Complete loss of activity	Acidithiobacillus ferrooxidans
N59A	mutation in the absolutely conserved KANRS motif. About 0.2% of wild-type activity	Neisseria meningitidis
N59DELTA	mutation in the absolutely conserved KANRS motif. Less than 0.1% of wild-type activity	Neisseria meningitidis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0025	-	D-arabinose 5-phosphate	wild-type, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
0.0029	-	phosphoenolpyruvate	mutant N57A, pH 7.5, temperature not specified in the publication	Acidithiobacillus ferrooxidans
0.0079	-	D-arabinose 5-phosphate	mutant N59A, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
0.012	-	phosphoenolpyruvate	wild-type, pH 7.5, temperature not specified in the publication	Acidithiobacillus ferrooxidans
0.012	-	D-arabinose 5-phosphate	wild-type, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
0.022	-	D-arabinose 5-phosphate	wild-type, pH 7.5, temperature not specified in the publication	Acidithiobacillus ferrooxidans
0.043	-	D-arabinose 5-phosphate	mutant N59DELTA, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
0.081	-	D-arabinose 5-phosphate	mutant N57A, pH 7.5, temperature not specified in the publication	Acidithiobacillus ferrooxidans
0.23	-	2-deoxy-D-ribose 5-phosphate	wild-type, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
0.432	-	2-deoxy-D-ribose 5-phosphate	mutant N59A, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
0.503	-	D-arabinose 5-phosphate	mutant N59A, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
1.1	-	D-arabinose 5-phosphate	mutant N59DELTA, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
1.5	-	2-deoxy-D-ribose 5-phosphate	mutant N57A, pH 7.5, temperature not specified in the publication	Acidithiobacillus ferrooxidans
5	-	2-deoxy-D-ribose 5-phosphate	wild-type, pH 7.5, temperature not specified in the publication	Acidithiobacillus ferrooxidans

Organism

Organism	UniProt	Comment	Textmining
Acidithiobacillus ferrooxidans	-	-	-
Neisseria meningitidis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate	-	Acidithiobacillus ferrooxidans	? + phosphate	-	?
phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate	-	Neisseria meningitidis	? + phosphate	-	?
phosphoenolpyruvate + D-arabinose 5-phosphate	-	Acidithiobacillus ferrooxidans	2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate	-	?
phosphoenolpyruvate + D-arabinose 5-phosphate	-	Neisseria meningitidis	2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.007	-	2-deoxy-D-ribose 5-phosphate	mutant N59A, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
0.13	-	2-deoxy-D-ribose 5-phosphate	wild-type, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
0.149	-	D-arabinose 5-phosphate	mutant N59A, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
0.178	-	D-arabinose 5-phosphate	mutant N59DELTA, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
0.202	-	2-deoxy-D-ribose 5-phosphate	mutant N57A, pH 7.5, temperature not specified in the publication	Acidithiobacillus ferrooxidans
0.3	-	D-arabinose 5-phosphate	mutant N57A, pH 7.5, temperature not specified in the publication	Acidithiobacillus ferrooxidans
0.66	-	2-deoxy-D-ribose 5-phosphate	wild-type, pH 7.5, temperature not specified in the publication	Acidithiobacillus ferrooxidans
4.8	-	D-arabinose 5-phosphate	wild-type, pH 7.5, temperature not specified in the publication	Acidithiobacillus ferrooxidans
8	-	D-arabinose 5-phosphate	wild-type, pH 7.5, temperature not specified in the publication	Neisseria meningitidis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.016	-	2-deoxy-D-ribose 5-phosphate	mutant N59A, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
0.016	-	D-arabinose 5-phosphate	mutant N59DELTA, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
0.13	-	2-deoxy-D-ribose 5-phosphate	wild-type, pH 7.5, temperature not specified in the publication	Acidithiobacillus ferrooxidans
0.13	-	2-deoxy-D-ribose 5-phosphate	mutant N57A, pH 7.5, temperature not specified in the publication	Acidithiobacillus ferrooxidans
0.29	-	D-arabinose 5-phosphate	mutant N59A, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
0.57	-	2-deoxy-D-ribose 5-phosphate	wild-type, pH 7.5, temperature not specified in the publication	Neisseria meningitidis
3.7	-	D-arabinose 5-phosphate	mutant N57A, pH 7.5, temperature not specified in the publication	Acidithiobacillus ferrooxidans
220	-	D-arabinose 5-phosphate	wild-type, pH 7.5, temperature not specified in the publication	Acidithiobacillus ferrooxidans
660	-	D-arabinose 5-phosphate	wild-type, pH 7.5, temperature not specified in the publication	Neisseria meningitidis

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Release 2023.1 (January 2023)