Cloned (Comment) | Organism |
---|---|
recombinant alpha- and beta-subunits, coexpressed in Escherichia coli, form a heterodimer and show activity to transfer farnesyl using farnesylpyrophosphate as a donor to human H-Ras possessing a C-terminal CVLS, but not a mutant H-Ras possessing CVLL. | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35600 | - |
1 * 35600 + 1 * 42600, calculation from nucleotide sequence | Entamoeba histolytica |
38000 | - |
1 * 38000 + 1 * 43000, SDS-PAGE | Entamoeba histolytica |
42600 | - |
1 * 35600 + 1 * 42600, calculation from nucleotide sequence | Entamoeba histolytica |
43000 | - |
1 * 38000 + 1 * 43000, SDS-PAGE | Entamoeba histolytica |
80000 | - |
gel filtration | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | Q76L89 | subunit Ftbeta | - |
Entamoeba histolytica | Q76L90 | subunit Ftalpha | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + human recombinant wild-type H-Ras-CVLS | - |
Entamoeba histolytica | diphosphate + ? | - |
? | |
farnesyl diphosphate + mutant H-Ras-CVLL | 20fold less active than human recombinant wild-type H-Ras-CVLS | Entamoeba histolytica | diphosphate + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 35600 + 1 * 42600, calculation from nucleotide sequence | Entamoeba histolytica |
dimer | 1 * 38000 + 1 * 43000, SDS-PAGE | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
EhFT | - |
Entamoeba histolytica |