KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
Ser-Ser-Cys-Ile-Leu-Leu | pH 7.4, 30°C | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
1 * 48000 + 1 * 40000 | Bos taurus |
48000 | - |
1 * 48000 + 1 * 40000 | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + protein-cysteine | Bos taurus | - |
S-geranylgeranyl-protein + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
peptide affinity chromatography of enzyme on SSCILL-Sepharose | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate | enzyme and geranylgeranyl diphosphate produce a stable binary complex and the tightly bound geranylgeranyl diphosphate is transferred to a peptide acceptor, without the equilibration of enzyme-bound geranylgeranyl diphosphate with free geranylgeranyl diphosphate | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0059 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu | ECB-gamma6 | Bos taurus | diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFF-S-farnesyl-CAI-Leu | - |
? | |
geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-ALEPPETEPKRKCCIF | ECB-G25K, 42% activity in comparison of protein substrate motif: CAA-leucine | Bos taurus | diphosphate + (biotin-CONH-(CH2)5-CO-)-ALEPPETEPKRK-S-geranylgeranyl-CCIF | - |
? | |
geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-GTPRASNRSCAIS | ECB-laminB(S), 5% activity in comparison of protein substrate motif: CAA-leucine | Bos taurus | diphosphate + (biotin-CONH-(CH2)5-CO-)-GTPRASNRS-S-geranylgeranyl-CAIS | - |
? | |
geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu | ECB-gamma6, preferred substrate | Bos taurus | diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFF-S-geranylgeranyl-CAI-Leu | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | - |
Bos taurus | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X. X is Leu, Phe or Ser, preferred substrate: Leu | Bos taurus | S-geranylgeranyl-protein + diphosphate | - |
? | |
Ser-Ser-Cys-Ile-Leu-Leu + geranylgeranyl diphosphate | - |
Bos taurus | diphosphate + Ser-Ser-S-geranylgeranyl-Cys-Ile-Leu-Leu | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 48000 + 1 * 40000 | Bos taurus |