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Literature summary for 2.5.1.59 extracted from

  • Zhang, F.L.; Moomaw, J.F.; Casey, P.J.
    Properties and kinetic mechanism of recombinant mammalian protein geranylgeranyltransferase type I (1994), J. Biol. Chem., 269, 23465-23470.
    View publication on PubMed
Search for more literature for 2.5.1.59

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of two subunits of enzyme by Sf9 cells infected with recombinant baculovirus Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
3-aza-geranylgeranyl-diphosphate competitive inhibitor with respect to geranylgeranyl diphosphate, non-competitive to Cys-Val-Phe-Leu Rattus norvegicus
Cys-Val-Phe-Leu noncompetitive inhibitor with respect to geranylgeranyl diphosphate, 50% inhibition at 0.0001 mM, competitive to Cys-Val-Phe-Leu Rattus norvegicus
additional information TKSer-Val-Ile-Leu inactive as competitor Rattus norvegicus
Thr-Lys-Cys-Val-Ile-Leu potent competitor, 50% inhibition at 0.001 mM Rattus norvegicus
Thr-Lys-Cys-Val-Ile-Met potent competitor, 50% inhibition at 0.008 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000003
-
 geranylgeranyl diphosphate pH 7.7, 30°C Rattus norvegicus
0.0012
-
 Ras-Cys-Val-Leu-Leu pH 7.7, 30°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ enzyme contains a single zinc atom Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 enzyme produced in Sf9cells using baculovirus system
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate random sequential mechanism Rattus norvegicus
a
geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate this enzyme, along with protein farnesyltransferase, EC 2.5.1.58 and protein geranylgeranyltransferase type II, EC 2.5.1.60, constitutes the protein prenyltransferase family of enzymes Rattus norvegicus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.007
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + protein-cysteine prenylation, substrates are Rho, Rac, most trimeric G protein gamma subunits Rattus norvegicus S-geranylgeranyl-protein + diphosphate
-
 ?
geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X. X is Leu, Phe or Ser, preferred substrate: Leu Rattus norvegicus S-geranylgeranyl-protein + diphosphate
-
 ?
geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X Rattus norvegicus S-geranylgeranyl-protein + diphosphate
-
 ?
geranylgeranyl diphosphate + Ras-Cys-Val-Leu-Leu
-
 Rattus norvegicus diphosphate + Ras-S-geranylgeranyl-Cys-Val-Leu-Leu
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 geranylgeranyl diphosphate
-
 Rattus norvegicus
0.02
-
 Ras-Cys-Val-Leu-Leu
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000015
-
 3-aza-geranylgeranyl-diphosphate with respect to geranylgeranyl diphosphate Rattus norvegicus
0.000022
-
 3-aza-geranylgeranyl-diphosphate with respect to Ras-Cys-Val-Leu-Leu Rattus norvegicus
0.00005
-
 Cys-Val-Phe-Leu with respect to Ras-Cys-Val-Leu-Leu Rattus norvegicus
0.000065
-
 Cys-Val-Phe-Leu with respect to geranylgeranyl diphosphate Rattus norvegicus