Cloned (Comment) | Organism |
---|---|
PCR-amplification of human enzyme for RT-PCR analysis of expression in human trabecular mesh cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GGTI-DU40 | 1-40 microM dissolved in dimethyl sulfoxide, affects actin cytoskeletal integrity, cell adhesion, cell-cell junctions, myosin II phosphorylation, and membrane localization of GTP-binding proteins in trabecular meshwork cells is tested using immunofluorescence detection and immunoblotting analysis and the effect on aqueous humor outflow | Homo sapiens | |
GGTI-DU40 | 5-40 microM dissolved in dimethyl sulfoxide influence cell morphology in a dose and time dependent manner, affects actin cytoskeletal integrity, cell adhesion, cell-cell junctions, myosin II phosphorylation, and membrane localization of GTP-binding proteins in trabecular meshwork cells is tested using immunofluorescence detection and immunoblotting analysis and the effect on aqueous humor outflow (25 and 40 microM) | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + Ras protein | Homo sapiens | - |
S-geranylgeranyl-Ras protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + Ras protein | Sus scrofa | - |
S-geranylgeranyl-Ras protein + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
eye | human trabecular meshwork cells, lysate for activity assay | Homo sapiens | - |
eye | porcine trabecular meshwork cells, freshly isolated, and organ culture | Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + Ras protein | - |
Homo sapiens | S-geranylgeranyl-Ras protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + Ras protein | - |
Sus scrofa | S-geranylgeranyl-Ras protein + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GGTase-I | - |
Homo sapiens |
GGTase-I | - |
Sus scrofa |
protein geranylgeranyltransferase type I | - |
Homo sapiens |
protein geranylgeranyltransferase type I | - |
Sus scrofa |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00007 | - |
complete inhibition at 5 microM, tritium-labelled geranylgeranyl diphosphate for activity assay with lysate of trabecular mesh cells, 30°C | Sus scrofa | GGTI-DU40 |