Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15(Qiagen) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
L-methionine | one kinetic form of alpha-two subunit in the presence of beta subunit | Homo sapiens | |
0.03 | 0.038 | L-methionine | one kinetic form of alpha subunit in the presence of beta subunit | Escherichia coli | |
0.06 | 0.1 | L-methionine | alpha-two subunit | Homo sapiens | |
0.065 | 0.08 | L-methionine | alpha subunit at low L-methionine concentrations | Escherichia coli | |
0.076 | - |
L-methionine | one kinetic form of alpha-two subunit in the presence of beta subunit | Homo sapiens | |
0.08 | 0.09 | L-methionine | alpha subunit at high L-methionine concentrations. Also one kinetic form of alpha subunit in the presence of beta subunit | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | 39000 | MAT II beta subunit, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Homo sapiens | Q9NZL9 | MAT-II | - |
Purification (Comment) | Organism |
---|---|
two methods: the first involved purification of the His-tagged protein under denaturing conditions of 8M urea and the second involved separation of SDS-PAGE | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Escherichia coli | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
ATP + L-methionine + H2O | - |
Homo sapiens | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | - |
Homo sapiens |
More | beta subunit has a regulatory function. MAT II beta subunit associates spontaneously with Escherichia coli MAT alpha subunit as well as with the recombinant human MAT II alpha-two subunit | Homo sapiens |