General Stability | Organism |
---|---|
enzyme is most stable in 0.3 M LiBr, 0.1 M 2-mercaptoethanol, 30% glycerol, 0.15 M Tris chloride, pH 8.0 to 9.0, or 0.3 M (NH4)2SO4 | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | CaCl2 | Cereibacter sphaeroides | |
Fe2+ | 0.1 mM FeSO4 | Cereibacter sphaeroides | |
Fe3+ | 0.1 mM FeCl3 | Cereibacter sphaeroides | |
Hg2+ | HgCl2, strong | Cereibacter sphaeroides | |
Iodine | - |
Cereibacter sphaeroides | |
Mg2+ | MgCl2 | Cereibacter sphaeroides | |
Mn2+ | MnCl2 | Cereibacter sphaeroides | |
N-ethylmaleimide | - |
Cereibacter sphaeroides | |
p-chloromercuribenzoate | strong | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
Hydroxymethylbilane | - |
Cereibacter sphaeroides | |
0.02 | - |
porphobilinogen | 0.1 mM porphobilinogen and 5 mM NaBH4 | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35500 | - |
gel filtration | Cereibacter sphaeroides |
36000 | - |
1 * 36000, SDS-PAGE in the presence of beta-mercaptoethanol | Cereibacter sphaeroides |
39000 | - |
sucrose density gradient centrifugation | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
porphobilinogen | Cereibacter sphaeroides | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
ATCC 11167 | - |
Purification (Comment) | Organism |
---|---|
- |
Cereibacter sphaeroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Cereibacter sphaeroides |
Storage Stability | Organism |
---|---|
stored in 0.1 M Tris chloride, pH 8.5, containing 30% glycerol and 0.01 M 2-mercaptoethanol. Under these conditions 50% loss of activity after 3 weeks | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Cereibacter sphaeroides | uroporphyrinogen III + 4 NH3 | - |
? | |
4 porphobilinogen + H2O | - |
Cereibacter sphaeroides | hydroxylmethylbilane + 4 NH3 | - |
? | |
porphobilinogen | - |
Cereibacter sphaeroides | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE in the presence of beta-mercaptoethanol | Cereibacter sphaeroides |
Synonyms | Comment | Organism |
---|---|---|
porphobilinogen deaminase | - |
Cereibacter sphaeroides |
uroporphyrinogen I synthetase | - |
Cereibacter sphaeroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cereibacter sphaeroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
during early stages of purification enzyme may be heated with no loss of activity. Inactivation is quite rapid above | Cereibacter sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
phosphate buffer and Tris buffer | Cereibacter sphaeroides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH 6.0: enzyme is rapidly and irreversibly inactivated below, pH 8.0: about 50% of maximal activity | Cereibacter sphaeroides |