Literature summary for 2.5.1.65 extracted from

Jurgenson, C.T.; Burns, K.E.; Begley, T.P.; Ealick, S.E.
Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of Mycobacterium tuberculosis (2008), Biochemistry, 47, 10354-10364.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as a His-tagged fusion protein	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
the structure of the protein complex CysM-CysO is determined at 1.53 A resolution. The protein complex in the crystal structure is asymmetric with one CysO (sulfur carrier protein) protomer binding to one end of a CysM dimer. The structures of CysM is determined individually at 2.8 A resolution. Sequence alignments with homologues and structural comparisons with CysK, a cysteine synthase that does not utilize a sulfur carrier protein, reveal high conservation of active site residues, but residues in CysM responsible for CysO binding are not conserved	Mycobacterium tuberculosis

Crystallization (Comment)

Organism

the structure of the protein complex CysM-CysO is determined at 1.53 A resolution. The protein complex in the crystal structure is asymmetric with one CysO (sulfur carrier protein) protomer binding to one end of a CysM dimer. The structures of CysM is determined individually at 2.8 A resolution. Sequence alignments with homologues and structural comparisons with CysK, a cysteine synthase that does not utilize a sulfur carrier protein, reveal high conservation of active site residues, but residues in CysM responsible for CysO binding are not conserved

Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
K204A	to improve crystallization of CysM alone, a putative surface residue in CysM (Lys204) is mutated to alanine using site-directed mutagenesis	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Mycobacterium tuberculosis

Subunits

Subunits	Comment	Organism
dimer	crystal structure	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
CysM	-	Mycobacterium tuberculosis
Rv1336	-	Mycobacterium tuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Mycobacterium tuberculosis