Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Enterobacter cloacae |
Crystallization (Comment) | Organism |
---|---|
in complex with substrates, hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 5.5), 0.2 M NH4OAc, 25% (w/v) PEG 3350 | Enterobacter cloacae |
Protein Variants | Comment | Organism |
---|---|---|
C115D | the mutant enzyme lacks the ability to react with phosphoenolpyruvate covalently | Enterobacter cloacae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosfomycin | - |
Enterobacter cloacae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | P33038 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Q-Sepharose column chromatography, Superdex 200 gel filtration, and Sephadex G-25 gel filtration | Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme does not display appreciable catalytic activity with UDP-N-acetyl-D-galactosamine as a substrate | Enterobacter cloacae | ? | - |
? | |
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Enterobacter cloacae | UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
MurA | - |
Enterobacter cloacae |
UDP-N-acetylglucosamine 1-carboxyvinyltransferase | - |
Enterobacter cloacae |