Cloned (Comment) | Organism |
---|---|
- |
Sophora flavescens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimeric enzymes between narigenin 8-dimethylallyltransferase and isoflavone-specific prenyltransferase G6DT responsible for the prenylation of the genistein at the 6-position, by recombination of amino acid sequences within the loop domains. Chimera SfG2N possesses purely narigenin 8-dimethylallyltransferase activity despite the absence of the N-terminal sequence derived from narigenin 8-dimethylallyltransferase, 243 amino acids. Chimera SfG3N possesses only isoflavone-specific prenyltransferase G6DT activity, although its 33% of the C-terminus is from narigenin 8-dimethylallyltransferase polypeptide. Neither chimeric enzyme can catalyze the prenylation reaction for other types of flavonoid such as kaempferol, apigenin, naringenin chalcone | Sophora flavescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
3,3-dimethylallyl diphosphate | pH 9.0, 30°C | Sophora flavescens | |
0.05 | - |
(2S)-naringenin | pH 9.0, 30°C | Sophora flavescens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Sophora flavescens | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sophora flavescens | F8WPJ0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3-dimethylallyl diphosphate + (2S)-liquiritigenin | - |
Sophora flavescens | diphosphate + isobavachin | - |
? | |
3,3-dimethylallyl diphosphate + (2S)-naringenin | - |
Sophora flavescens | diphosphate + sophoraflavanone B | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N8DT-3 | - |
Sophora flavescens |