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Literature summary for 2.5.1.76 extracted from

  • Graham, D.E.; Taylor, S.M.; Wolf, R.Z.; Namboori, S.C.
    Convergent evolution of coenzyme M biosynthesis in the Methanosarcinales: cysteate synthase evolved from an ancestral threonine synthase (2009), Biochem. J., 424, 467-478.
    View publication on PubMed
Search for more literature for 2.5.1.76

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanosarcina acetivorans

Protein Variants

Protein Variants Comment Organism
additional information expression of truncated protein lacking the first 43 codons of the gene results in an insoluble protein. The refolded protein catalyzes the elimination of phosphate from L-phosphoserine with a rate of 3% of the full-length protein. It forms a monomer of 42300 Da instead of the trimer of the native protein Methanosarcina acetivorans

Inhibitors

Inhibitors Comment Organism Structure
DL-2-amino-3-phosphonopropionate
-
 Methanosarcina acetivorans
DL-2-Amino-4-phosphonobutanoate
-
 Methanosarcina acetivorans
DL-2-amino-5-phosphonopentanoate 1 mM, more than 80% reemaining activity Methanosarcina acetivorans
L-aspartate
-
 Methanosarcina acetivorans
L-cysteate
-
 Methanosarcina acetivorans
L-lysine 1 mM, more than 80% reemaining activity Methanosarcina acetivorans
L-serine
-
 Methanosarcina acetivorans
L-threonine 1 mM, more than 80% reemaining activity Methanosarcina acetivorans
additional information not inhibitory: L-glutamate, D-glutamate, L-glutamine or L-homoserine Methanosarcina acetivorans
O-acetyl-L-serine 1 mM, more than 80% reemaining activity Methanosarcina acetivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
 L-phosphoserine pH 7.0, 37°C Methanosarcina acetivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 3 * 46000, SDS-PAGE Methanosarcina acetivorans
132000
-
 gel filtration Methanosarcina acetivorans

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans Q8TKU7
-
-

Renatured (Commentary)

Renatured (Comment) Organism
expression of truncated protein lacking the first 43 codons of the gene results in an insoluble protein. The refolded protein catalyzes the elimination of phosphate from L-phosphoserine with a rate of 3% of the full-length protein Methanosarcina acetivorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.015
-
 pH 7.0, 37°C Methanosarcina acetivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phosphoserine + sulfite
-
 Methanosarcina acetivorans L-cysteate + phosphate
-
 ?
additional information no reaction in absence of sulfite, no cysteine synthase activity. No substrates are: thiosulfate, nitrite, cyanide or thiocyanide anions Methanosarcina acetivorans ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 46000, SDS-PAGE Methanosarcina acetivorans

Synonyms

Synonyms Comment Organism
MA3297
-
 Methanosarcina acetivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
 L-phosphoserine pH 7.0, 37°C Methanosarcina acetivorans

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14
-
 pH 7.0, 37°C Methanosarcina acetivorans DL-2-amino-3-phosphonopropionate
12.6
-
 pH 7.0, 37°C Methanosarcina acetivorans L-cysteate
15.9
-
 pH 7.0, 37°C Methanosarcina acetivorans L-aspartate
16.5
-
 pH 7.0, 37°C Methanosarcina acetivorans DL-2-Amino-4-phosphonobutanoate
37.1
-
 pH 7.0, 37°C Methanosarcina acetivorans L-serine