Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Brucella abortus |
expression in Escherichia coli | Brucella melitensis |
expression in Escherichia coli | Mesorhizobium loti |
Crystallization (Comment) | Organism |
---|---|
isoform RibH1, bound to the substrate analogue inhibitor 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. Comparison with structure of isoform RibH2 | Brucella melitensis |
isoform RibH1, unliganded, to 2.2 A resolution, and bound to the substrate analogue inhibitor 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. Comparison with structure of isoform RibH2 | Brucella abortus |
isoform RibH2, bound to the substrate analogue inhibitor 5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | Mesorhizobium loti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | crystallization data | Brucella abortus | |
5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | crystallization data | Brucella melitensis | |
5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | crystallization data; crystallization data | Mesorhizobium loti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | isoform RibH1, pH 7.0, 37°C | Mesorhizobium loti | |
0.004 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C | Brucella melitensis | |
0.015 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | isoform RibH1, pH 7.0, 37°C | Mesorhizobium loti | |
0.02 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | isoform RibH2, pH 7.0, 37°C | Mesorhizobium loti | |
0.225 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | pH 7.0, 37°C | Brucella melitensis | |
0.45 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | isoform RibH2, pH 7.0, 37°C | Mesorhizobium loti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16800 | - |
5 * 16800, calculated, isoform RibH1 | Brucella abortus |
16800 | - |
5 * 16800, calculated, isoform RibH1 | Brucella melitensis |
17200 | - |
5 * 17200, calculated, isoform RibH1 | Mesorhizobium loti |
17300 | - |
10 * 17300, calculated, isoform RibH2 | Mesorhizobium loti |
75300 | - |
isoform RibH1, light scattering experiments | Brucella melitensis |
80100 | - |
light scattering experiments, isoform RibH1 | Brucella abortus |
85500 | - |
isoform RibH1, light scattering experiments | Mesorhizobium loti |
158500 | - |
isoform RinH2, light scattering experiments | Mesorhizobium loti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brucella abortus | - |
- |
- |
Brucella melitensis | - |
isoform RibH1 | - |
Mesorhizobium loti | Q983B0 | - |
- |
Mesorhizobium loti | Q986N2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | - |
Brucella melitensis | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | - |
Mesorhizobium loti | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione + L-3,4-dihydroxybutan-2-one 4-phosphate | - |
Mesorhizobium loti | 6,7-dimethyl-8-(1-D-ribityl)lumazine + 2 H2O + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 17300, calculated, isoform RibH2 | Mesorhizobium loti |
pentamer | 5 * 16800, calculated, isoform RibH1 | Brucella abortus |
pentamer | 5 * 16800, calculated, isoform RibH1 | Brucella melitensis |
pentamer | 5 * 17200, calculated, isoform RibH1 | Mesorhizobium loti |
Synonyms | Comment | Organism |
---|---|---|
6,7-dimethyl-8-ribityllumazine synthase | - |
Brucella abortus |
ribH1 | - |
Mesorhizobium loti |
RibH2 | - |
Mesorhizobium loti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Brucella melitensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | isoform RibH2, pH 7.0, 37°C | Mesorhizobium loti | |
0.003 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | pH 7.0, 37°C | Brucella melitensis | |
0.04 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | isoform RibH1, pH 7.0, 37°C | Mesorhizobium loti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Brucella melitensis |