Crystallization (Comment) | Organism |
---|---|
crystals are grown at 18°C by the sitting drop vapor diffusion method. The W27Y mutant protein in complex with riboflavin, the substrate analogue 5-nitroso-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, and the product analogue 6-carboxyethyl-7-oxo-8-ribityllumazine, are determined by X-ray crystallography at resolutions of 2.72.8 A | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
W27Y | whereas the indole system of W27 forms a coplanar pi-complex with riboflavin, the corresponding phenyl ring in the W27Y mutant establishes only peripheral contact with the heterocyclic ring system of the bound riboflavin | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-nitro-6-(D-ribitylamino)-2,4(1H,3H)-pyrimidinedione | - |
Schizosaccharomyces pombe | |
5-nitroso-6-(D-ribitylamino)-2,4(1H,3H)-pyrimidinedione | - |
Schizosaccharomyces pombe | |
6-carboxyethyl-7-oxo-8-ribityllumazine | - |
Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | Schizosaccharomyces pombe | - |
6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | - |
Schizosaccharomyces pombe | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6,7-dimethyl-8-ribityllumazine synthase | - |
Schizosaccharomyces pombe |