Application | Comment | Organism |
---|---|---|
medicine | the fact that the enzymes of the riboflavin biosynthesis pathway are not present in the human or animal host makes them potential targets for anti-infective agents | Candida albicans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Candida albicans |
Crystallization (Comment) | Organism |
---|---|
crystallized in sitting drops by vapor diffusion. The crystal structure of lumazine synthase from Candida albicans is solved by molecular replacement and refined at 2.5 A resolution. The results of crystallographic investigations and sedimentation equilibrium experiments clearly indicate the presence of pentameric assemblies of the enzyme either in crystals or in solution | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,3,7-trihydro-9-D-ribityl-2,4,8-purinetrione | association constants and thermodynamic parameters of binding of different inhibitors to lumazine synthase | Candida albicans | |
3-(1,3-dihydro-9-D-ribityl-2,4,8-purinetrione-7-yl)propane 1-phosphate | association constants and thermodynamic parameters of binding of different inhibitors to lumazine synthase | Candida albicans | |
4-(6,7(5H,8H)-dioxo-8-D-ribityllumazine-5-yl)butane 1-phosphate | association constants and thermodynamic parameters of binding of different inhibitors to lumazine synthase | Candida albicans | |
4-(6-chloro-2,4-dioxo-1,2,3,4-tetrahydropyrimidine-5-yl)-butyl phosphate | association constants and thermodynamic parameters of binding of different inhibitors to lumazine synthase | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | Candida albicans | the enzyme is involved in riboflavin biosynthesis in many plants and microorganisms | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Candida albicans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.166 | - |
- |
Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | - |
Candida albicans | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | the enzyme is involved in riboflavin biosynthesis in many plants and microorganisms | Candida albicans | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? |