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Literature summary for 2.5.1.80 extracted from

  • Yu, X.; Li, S.M.
    Prenylation of flavonoids by using a dimethylallyltryptophan synthase, 7-DMATS, from Aspergillus fumigatus (2011), ChemBioChem, 12, 2280-2283.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
7-DMATS expression in Escherichia coli Aspergillus fumigatus

Inhibitors

Inhibitors Comment Organism Structure
phloretin shows substrate inhibition at 1 mM or higher concentrations Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
Biochanin A pH not specified in the publication, 37°C Aspergillus fumigatus
0.13
-
phloretin pH not specified in the publication, 37°C Aspergillus fumigatus
0.14
-
L-tryptophan pH and temperature not specified in the publication Aspergillus fumigatus
0.16
-
genistein pH not specified in the publication, 37°C Aspergillus fumigatus
0.99
-
naringenin pH not specified in the publication, 37°C Aspergillus fumigatus
1.1
-
hesperetin pH not specified in the publication, 37°C Aspergillus fumigatus
1.26
-
eriodictyol pH not specified in the publication, 37°C Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + L-tryptophan Aspergillus fumigatus
-
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + apigenin
-
Aspergillus fumigatus diphosphate + ?
-
?
dimethylallyl diphosphate + biochanin A 15.9% activity compared to substrate L-tryptophan Aspergillus fumigatus diphosphate + gancaonin A
-
?
dimethylallyl diphosphate + eriodictyol 19.0% activity compared to substrate L-tryptophan Aspergillus fumigatus diphosphate + 6-(3-methylbut-2-enyl)eriodictyol
-
?
dimethylallyl diphosphate + genistein 10.4% activity compared to substrate L-tryptophan Aspergillus fumigatus diphosphate + wighteone
-
?
dimethylallyl diphosphate + hesperetin 1.4% activity compared to substrate L-tryptophan Aspergillus fumigatus diphosphate + sigmoidin B
-
?
dimethylallyl diphosphate + L-tryptophan
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
?
dimethylallyl diphosphate + L-tryptophan L-tryptophan is the preferred substrate Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
?
dimethylallyl diphosphate + luteolin
-
Aspergillus fumigatus diphosphate + ?
-
?
dimethylallyl diphosphate + naringenin 1.4% activity compared to substrate L-tryptophan Aspergillus fumigatus diphosphate + 6-(3-methylbut-2-enyl)naringenin
-
?
dimethylallyl diphosphate + phloretin 17.4% activity compared to substrate L-tryptophan Aspergillus fumigatus diphosphate + 6-dimethylallyl-5,7,9,1'-tetrahydroxydihydrochalcone
-
?
additional information 7-DMATS substrate specificity with flavonoid compounds, overview. Chalcones, isoflavonoids, and flavanones are much better accepted than flavones and flavonols Aspergillus fumigatus ?
-
?

Synonyms

Synonyms Comment Organism
7-DMATS
-
Aspergillus fumigatus
dimethylallyltryptophan synthase
-
Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.019
-
Biochanin A pH not specified in the publication, 37°C Aspergillus fumigatus
0.023
-
naringenin pH not specified in the publication, 37°C Aspergillus fumigatus
0.026
-
hesperetin pH not specified in the publication, 37°C Aspergillus fumigatus
0.027
-
genistein pH not specified in the publication, 37°C Aspergillus fumigatus
0.036
-
phloretin pH not specified in the publication, 37°C Aspergillus fumigatus
0.23
-
L-tryptophan pH and temperature not specified in the publication Aspergillus fumigatus
0.39
-
eriodictyol pH not specified in the publication, 37°C Aspergillus fumigatus

General Information

General Information Comment Organism
evolution the enzyme belongs to the DMATS superfamily, 7-DMATS has a more flexible substrate specificity towards flavonoid compounds than other fungal prenyltransferases Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.023
-
naringenin pH not specified in the publication, 37°C Aspergillus fumigatus
0.024
-
hesperetin pH not specified in the publication, 37°C Aspergillus fumigatus
0.171
-
genistein pH not specified in the publication, 37°C Aspergillus fumigatus
0.261
-
Biochanin A pH not specified in the publication, 37°C Aspergillus fumigatus
0.286
-
phloretin pH not specified in the publication, 37°C Aspergillus fumigatus
0.312
-
eriodictyol pH not specified in the publication, 37°C Aspergillus fumigatus
1.643
-
L-tryptophan pH and temperature not specified in the publication Aspergillus fumigatus