Cloned (Comment) | Organism |
---|---|
7-DMATS expression in Escherichia coli | Aspergillus fumigatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phloretin | shows substrate inhibition at 1 mM or higher concentrations | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
Biochanin A | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
0.13 | - |
phloretin | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
0.14 | - |
L-tryptophan | pH and temperature not specified in the publication | Aspergillus fumigatus | |
0.16 | - |
genistein | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
0.99 | - |
naringenin | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
1.1 | - |
hesperetin | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
1.26 | - |
eriodictyol | pH not specified in the publication, 37°C | Aspergillus fumigatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tryptophan | Aspergillus fumigatus | - |
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + apigenin | - |
Aspergillus fumigatus | diphosphate + ? | - |
? | |
dimethylallyl diphosphate + biochanin A | 15.9% activity compared to substrate L-tryptophan | Aspergillus fumigatus | diphosphate + gancaonin A | - |
? | |
dimethylallyl diphosphate + eriodictyol | 19.0% activity compared to substrate L-tryptophan | Aspergillus fumigatus | diphosphate + 6-(3-methylbut-2-enyl)eriodictyol | - |
? | |
dimethylallyl diphosphate + genistein | 10.4% activity compared to substrate L-tryptophan | Aspergillus fumigatus | diphosphate + wighteone | - |
? | |
dimethylallyl diphosphate + hesperetin | 1.4% activity compared to substrate L-tryptophan | Aspergillus fumigatus | diphosphate + sigmoidin B | - |
? | |
dimethylallyl diphosphate + L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | L-tryptophan is the preferred substrate | Aspergillus fumigatus | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + luteolin | - |
Aspergillus fumigatus | diphosphate + ? | - |
? | |
dimethylallyl diphosphate + naringenin | 1.4% activity compared to substrate L-tryptophan | Aspergillus fumigatus | diphosphate + 6-(3-methylbut-2-enyl)naringenin | - |
? | |
dimethylallyl diphosphate + phloretin | 17.4% activity compared to substrate L-tryptophan | Aspergillus fumigatus | diphosphate + 6-dimethylallyl-5,7,9,1'-tetrahydroxydihydrochalcone | - |
? | |
additional information | 7-DMATS substrate specificity with flavonoid compounds, overview. Chalcones, isoflavonoids, and flavanones are much better accepted than flavones and flavonols | Aspergillus fumigatus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
7-DMATS | - |
Aspergillus fumigatus |
dimethylallyltryptophan synthase | - |
Aspergillus fumigatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aspergillus fumigatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
Biochanin A | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
0.023 | - |
naringenin | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
0.026 | - |
hesperetin | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
0.027 | - |
genistein | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
0.036 | - |
phloretin | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
0.23 | - |
L-tryptophan | pH and temperature not specified in the publication | Aspergillus fumigatus | |
0.39 | - |
eriodictyol | pH not specified in the publication, 37°C | Aspergillus fumigatus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the DMATS superfamily, 7-DMATS has a more flexible substrate specificity towards flavonoid compounds than other fungal prenyltransferases | Aspergillus fumigatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
naringenin | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
0.024 | - |
hesperetin | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
0.171 | - |
genistein | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
0.261 | - |
Biochanin A | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
0.286 | - |
phloretin | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
0.312 | - |
eriodictyol | pH not specified in the publication, 37°C | Aspergillus fumigatus | |
1.643 | - |
L-tryptophan | pH and temperature not specified in the publication | Aspergillus fumigatus |