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Literature summary for 2.5.1.80 extracted from
- Prenyltransferases of the dimethylallyltryptophan synthase superfamily (2012), Methods Enzymol., 516, 259-278.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | 7-DMATS catalyzes regio- and stereospecific prenylations and can be used as efficient catalysts for chemoenzymatic synthesis of prenylated compounds, which can be then tested for their biological activities in drug discovery and development programs | Aspergillus fischeri |
| drug development | 7-DMATS catalyzes regio- and stereospecific prenylations and can be used as efficient catalysts for chemoenzymatic synthesis of prenylated compounds, which can be then tested for their biological activities in drug discovery and development programs | Aspergillus clavatus |
| synthesis | 7-DMATS catalyzes regio- and stereospecific prenylations and can be used as efficient catalysts for chemoenzymatic synthesis of prenylated compounds | Aspergillus fischeri |
| synthesis | 7-DMATS catalyzes regio- and stereospecific prenylations and can be used as efficient catalysts for chemoenzymatic synthesis of prenylated compounds | Aspergillus clavatus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged 7-DMATS in Escherichia coli strains XL1 Blue or M15 or in Saccharomyces cerevisiae strain INVSc1 | Aspergillus fischeri |
| expression of His-tagged 7-DMATS in Escherichia coli strains XL1 Blue or M15 or in Saccharomyces cerevisiae strain INVSc1 | Aspergillus clavatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics analyis, overview | Aspergillus fischeri | |
| additional information | - |
additional information | kinetics analyis, overview | Aspergillus clavatus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Aspergillus fischeri |  |
| Ca2+ | required | Aspergillus clavatus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + L-tryptophan | Aspergillus fischeri | - |
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
| dimethylallyl diphosphate + L-tryptophan | Aspergillus clavatus | - |
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus clavatus | - |
- |
- |
| Aspergillus fischeri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged 7-DMATS from Escherichia coli strains XL1 Blue or M15 or from Saccharomyces cerevisiae strain INVSc1 by nickel affinity chromatography | Aspergillus fischeri |
| recombinant His-tagged 7-DMATS from Escherichia coli strains XL1 Blue or M15 or from Saccharomyces cerevisiae strain INVSc1 by nickel affinity chromatography | Aspergillus clavatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus clavatus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + L-tryptophan | - |
Aspergillus fischeri | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
| dimethylallyl diphosphate + L-tryptophan | - |
Aspergillus clavatus | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
| dimethylallyl diphosphate + L-tryptophan | 7-DMATS accept L-tryptophan as substrate and prenylate it at C-7 | Aspergillus fischeri | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
| dimethylallyl diphosphate + L-tryptophan | 7-DMATS accept L-tryptophan as substrate and prenylate it at C-7 | Aspergillus clavatus | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
| additional information | 7-DMATS catalyzes regio- and stereospecific prenylations, assay conditions with different substrates, detailed overview | Aspergillus fischeri | ? | - |
? | |
| additional information | 7-DMATS catalyzes regio- and stereospecific prenylations, assay conditions with different substrates, detailed overview | Aspergillus clavatus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 7-DMATS | - |
Aspergillus fischeri |
| 7-DMATS | - |
Aspergillus clavatus |
| dimethylallyltryptophan synthase | - |
Aspergillus fischeri |
| dimethylallyltryptophan synthase | - |
Aspergillus clavatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | assay at | Aspergillus fischeri |
| 30 | 37 | assay at | Aspergillus clavatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Aspergillus fischeri |
| 7.5 | - |
assay at | Aspergillus clavatus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the DMATS superfamily. The members of the DMATS superfamily contain no DDxxD motifs, which are essential for binding of prenyl diphosphate via metal ions, for example, Mg2+ or Mn2+, in trans prenyltransferases | Aspergillus fischeri |
| evolution | the enzyme belongs to the DMATS superfamily. The members of the DMATS superfamily contain no DDxxD motifs, which are essential for binding of prenyl diphosphate via metal ions, for example, Mg2+ or Mn2+, in trans prenyltransferases | Aspergillus clavatus |
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Release 2023.1 (January 2023)
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