Activating Compound | Comment | Organism | Structure |
---|---|---|---|
deoxycholate | microsomal enzyme and enzyme released from microsomes are maximally activated at 5 mM. In the absence of deoxycholate, the released enzyme is inactive, while the microsomal enzyme has significant activity | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | 0.4 M, more than 90% inhibition | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000532 | - |
isopentenyl diphosphate | pH 8.5, 37°C, enzyme released from microsomes | Sus scrofa | |
0.000538 | - |
isopentenyl diphosphate | pH 8.5, 37°C, microsomal enzyme | Sus scrofa | |
0.013 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, microsomal enzyme | Sus scrofa | |
0.0149 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, enzyme released from microsomes | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Sus scrofa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximal activity at 1 mM | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
gel filtration of the released enzyme gives a peak of dehydrodolichyl diphosphate synthase activity, which appears between 150000 Da and 50000 Da molecular mass markers | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Sus scrofa | - |
Storage Stability | Organism |
---|---|
-80°C, presence of glycerol is indispensable | Sus scrofa |
4°C, 5 days, 20% glycerol, more than 60% of enzyme activity is retained | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Sus scrofa | diphosphate + di-trans,poly-cis-polyprenyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dehydrodolichyl diphosphate synthase | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Sus scrofa |