Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and Saccharomyces cerevisiae | Escherichia coli K-12 |
Protein Variants | Comment | Organism |
---|---|---|
A79Y | no activity. Cells harboring wild-type ispB and the A79Y mutant produce mainly ubiquinone-6, although the activity of the enzyme with the A79Y mutation is completely abolished. Although the A79Y mutant is functionally inactive, it can regulate activity upon forming a heterodimer with wild-type IspB, and this dimer formation is important for the determination of the isoprenoid chain length | Escherichia coli K-12 |
F75A | resulting ubiquinone species are almost the same as those produced by the wild-type enzyme | Escherichia coli K-12 |
I32V | resulting ubiquinone species are almost the same as those produced by the wild-type enzyme | Escherichia coli K-12 |
K170A | mutant cannot be isolated, mutant protein does not retain functional activity | Escherichia coli K-12 |
K170G | mutant cannot be isolated, mutant protein does not retain functional activity | Escherichia coli K-12 |
K235L | main product is ubiquinone-8 | Escherichia coli K-12 |
L31V | resulting ubiquinone species are almost the same as those produced by the wild-type enzyme | Escherichia coli K-12 |
R321A | normal growth at 30°C, no growth at 43°C, main product is ubiquinone-8 | Escherichia coli K-12 |
R321D | normal growth at 30°C, no growth at 43°C | Escherichia coli K-12 |
R321V | products are ubiquinone-6 and ubiquinone-7, slow growth at 43°C | Escherichia coli K-12 |
Y37A | mutant cannot be isolated, mutant protein does not retain functional activity | Escherichia coli K-12 |
Y37A/Y38A | increased production of ubiquinone-6 | Escherichia coli K-12 |
Y38A | products are ubiquinone-6 and ubiquinone-7 | Escherichia coli K-12 |
Y38A/R321V | products are ubiquinone-5 and ubiquinone-6 | Escherichia coli K-12 |
Y61V | resulting ubiquinone species are almost the same as those produced by the wild-type enzyme | Escherichia coli K-12 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | P0AD57 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | residues Tyr-38, Lys-235, and Arg-321 are involved in chain length determination | Escherichia coli K-12 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
octaprenyl-diphosphate synthase | - |
- |