Literature summary for 2.5.1.90 extracted from

Guo, R.-T.; Kuo, C.-J.; Ko, T.-P.; Chou, C.-C.; Liang, P.-H.; Wang, A.H.J.
A molecular ruler for chain elongation catalyzed by octaprenyl pyrophosphate synthase and its structure-based engineering to produce unprecedented long chain trans-prenyl products (2004), Biochemistry, 43, 7678-7686.

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutants A76Y, A76Y/S77F, F132A/L128A, F132A/L128A/I123A, and F132A/L128A/I123A/D62A to 3.1, 2.7, 3.3, 3.35 and 3.4 A resolution, respectively. Like wildtype OPPs, all mutant structures contain 12 alpha-helices, nine of them surrounding a large central cavity and an elongated tunnel-shaped active site cavity surrounded by four alpha-helices In the crystal structure of the A76Y/S77F mutant, F77 is pushed away by Y76, thereby creating more space between those two large amino acids to accommodate the C20 product. A large F132 residue at the bottom of the tunnel-shaped active site serves as the floor and determines the final product chain length. The substitution of F132 with a small Ala, thereby removing the blockade, leads to the synthesis of a C50 product larger than that produced by the wild-type enzyme	Thermotoga maritima

Crystallization (Comment)

Organism

mutants A76Y, A76Y/S77F, F132A/L128A, F132A/L128A/I123A, and F132A/L128A/I123A/D62A to 3.1, 2.7, 3.3, 3.35 and 3.4 A resolution, respectively. Like wildtype OPPs, all mutant structures contain 12 alpha-helices, nine of them surrounding a large central cavity and an elongated tunnel-shaped active site cavity surrounded by four alpha-helices In the crystal structure of the A76Y/S77F mutant, F77 is pushed away by Y76, thereby creating more space between those two large amino acids to accommodate the C20 product. A large F132 residue at the bottom of the tunnel-shaped active site serves as the floor and determines the final product chain length. The substitution of F132 with a small Ala, thereby removing the blockade, leads to the synthesis of a C50 product larger than that produced by the wild-type enzyme

Thermotoga maritima

Protein Variants

Protein Variants	Comment	Organism
A76Y	mutant produces only C20 cores instead of the C40 core of octaprenyl diphosphate	Thermotoga maritima
A76Y/S77F	mutant produces only C20 cores instead of the C40 core of octaprenyl diphosphate. The A76Y/S77F mutant synthesizes a larger amount of C20 than the A76Y mutant	Thermotoga maritima
F132A	steady-state activity 0.0051 per s. Product chain length C45 to C60	Thermotoga maritima
F132A/L128A	steady-state activity 0.0008 per s. Product chain length C55, C60	Thermotoga maritima
F132A/L128A/I123A	steady-state activity 0.00066 per s. Produuct chain length C55 to C75	Thermotoga maritima
F132A/L128A/I123A/D62A	products reach C95, beyond the largest chain length generated by all known trans-prenyltransferases. Steady-state activity 0.00061 per s	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9X1M1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate	-	Thermotoga maritima	5 diphosphate + all-trans-octaprenyl diphosphate	-	?