Cloned (Comment) | Organism |
---|---|
the proteins Dps1 abd Dlp1 must be present simultaneously in Escherichia coli transformants before ubiquinone-10, which is produced by Schizosaccharomyces pombe but not by Escherichia coli, is generated. DLP1 and DPS1 form a heterotetramer in Escherichia coli | Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17500 | - |
gel filtration | Schizosaccharomyces pombe |
32411 | - |
2 * 42047 (DSP1) + 2 * 32411 (DLP1), both DPS1 and DLPd1 are required for the enzymatic activity, calculated from sequence | Schizosaccharomyces pombe |
42047 | - |
2 * 42047 (DSP1) + 2 * 32411 (DLP1), both DPS1 and DLPd1 are required for the enzymatic activity, calculated from sequence | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | O13851 | gene DLP1 | - |
Schizosaccharomyces pombe | O43091 | gene DPS1 | - |
Schizosaccharomyces pombe | O43091 and O13851 | O43091: subunit 1 (DSP1), O13851: subunit 2 (DLP1) | - |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 42047 (DSP1) + 2 * 32411 (DLP1), both DPS1 and DLPd1 are required for the enzymatic activity, calculated from sequence | Schizosaccharomyces pombe |
More | both Dps1 and Dlp1 are required for enzymatic activity | Schizosaccharomyces pombe |
tetramer | 2 * Dps1 + 2 * Dlp1, heterotetramer | Schizosaccharomyces pombe |
Synonyms | Comment | Organism |
---|---|---|
D-less polyprenyl diphosphate synthase | - |
Schizosaccharomyces pombe |
decaprenyl diphosphate synthase | - |
Schizosaccharomyces pombe |
DLP1 | - |
Schizosaccharomyces pombe |
DPS1 | - |
Schizosaccharomyces pombe |
General Information | Comment | Organism |
---|---|---|
malfunction | the deletion mutant of dlp1 lacks the enzymatic activity of decaprenyl diphosphate synthase, does not produce ubiquinone-10 and has the typical ubiquinone-deficient Schizosaccharomyces pombe phenotypes, namely hypersensitivity to hydrogen peroxide, the need for antioxidants for growth on minimal medium and an elevated production of H2S. Both the dps1 and dlp1 mutants can generate ubiquinone when they are transformed with a bacterial decaprenyl diphosphate synthase, which functions in its host as a homodimer | Schizosaccharomyces pombe |
physiological function | both genes DPS1 and DLP1 are required for decaprenyl diphosphate synthase activity. The deletion mutant of dlp1 lacks the enzymatic activity of decaprenyl diphosphate synthase, does not produce ubiquinone-10 and has the typical ubiquinone-deficient Schizosaccharomyces pombe phenotypes, namely hypersensitivity to hydrogen peroxide, the need for antioxidants for growth on minimal medium and an elevated production of H2S. Both the dps1 and dlp1 mutants can generate ubiquinone when they are transformed with a bacterial decaprenyl diphosphate synthase, which functions in its host as a homodimer | Schizosaccharomyces pombe |